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Thursday, December 15, 2011

How Bees Choose Home


The decision-making process of honeybee swarms mimics how our brains choose between two options.

By Tia Ghose | 
HoneybeesThomas SeeleyHoneybeesThomas Seeley
For honeybees, there’s no place like home. And every year, they must find a new one. Now, a study publishing today (December 8) in Science suggests that the honeybee swarms use inhibitory signals when house-hunting, paralleling the human brain’s decision-making process.
“It’s just another lovely example of the amazing sophistication in the honeybee population,” said University of Sussex apiculturist Francis Ratnieks, who was not involved in the study. It also shows a “commonality in the decision making processes between a brain and a swarm,” he added.
Every spring, about two-thirds of the honeybee colony  split off from the group to form a new swarm, but until they find a roomy, hollow tree to call home, the thousands of bees wait in a tree branch as a few hundred scouts explore new prospects, said mathematician Mary Myerscough of the University of Sydney in Australia, who was not involved in the study.
After canvassing one potential home site, each scout returns to the group and reports the quality of the site by doing a waggle dance on the swarm surface, literally dancing on a platform of bees. Individual bees don’t compare multiple sites, but visit only one and instinctively know the difference between a so-so spot and “a bee five-star mansion,” Myerscough said. The better the digs, the longer and more vivaciously they dance, thereby recruiting more bees to their site. The scout who recruits a certain threshold number of bees wins, and the swarm heads to that bee’s scouted location to set up shop.
But if two nests are equally cozy, the bees risk a deadly stalemate. The swarm has only one queen bee, after all, so it can’t split up, and thus must agree one a single nest site. To find out how they avoid deadlock, bee biologist Thomas Seeley of Cornell University and his colleagues set up two identical nest boxes on a remote Maine island, then released a swarm of honeybees. Scouts who visited one nest box were painted with a yellow stripe, while those who visited the others were painted pink.
Then, the researchers watched the scout bees jockey for one nest or the other. The scouts waggled for their sites, but they also took time out from dancing to stop other bees from doing their jigs—by head-butting them and emitting a high-pitched beep, Seeley said.
Using these inhibitory signals allows the bees to break deadlock and decide on a home faster, because once one site gains even the tiniest edge over the other, there are more bees on the winning nest side to stop the other side’s waggling, accelerating the inequality and allowing the bees to  choose a site more quickly. “It amplifies differences in the support for the two sites that are actually equal in quality,” Seeley said. While prior research had shown that bee head butting could warn bees away from risky foraging situations, this is the first time it’s been shown to hasten the consensus-reaching process.
The bees’ waggling standoffs parallel how primate brains process information, Ratnieks said. To determine which side a sound came from, for instance, neurons from the left and right ear might both initially fire. But neurons will also suppress the firing of neurons from the other ear, and once a threshold level is reached, the brain concludes that the sound came from the left.
The findings could also have implications for artificial intelligence and some crowd-sourced decision-making systems. In the US primary elections, for instance, votes are aggregated from many individuals to make a decision for the group, and only one candidate can prevail in the end, Seeley said. In theory that could potentially benefit from some built-in negative signaling, he added.
“That’s not something we humans are entirely fond of because we don’t really like negative campaigning,” he said.
T. Seeley, et al, “Stop Signals Provide Cross Inhibition in Collective Decision Making by Honeybee Swarms,” Science, doi: 10.1126/science.1210361, 2011.
Source: The Scientist
http://the-scientist.com/2011/12/08/how-bees-choose-home/
Posted by
Robert Karl Stonjek

The brain on trial



How should insights about the brain affect the course of a criminal trial, from the arguments in a courtroom to the issuing of a sentence?
This was the topic of the Fred Kavli Public Symposium, held recently at the Society for Neuroscience's "Neuroscience 2011." Titled "The Brain on Trial: Neuroscience and the Law," the symposium looked at how advances in neuroscience are both challenging and assisting the judicial system. To explore this further, The Kavli Foundation brought together three experts to discuss the subject. Joining the dialogue:
  • Alan Leshner, symposium chair; chief executive officer of the American Association for the Advancement of Science and former head of the National Institute of Drug Abuse;
  • Martha Farah, director of the Center for Neuroscience and Society, University of Pennsylvania;
  • Jay Giedd, MD, an expert in adolescent brain development at the National Institute of Mental Health and chief of NIMH's Unit on Brain Imaging in the Child Psychiatry Branch.
Together they examined the role neuroscience should have in determining legal policies and judgments, discussed innovative brain-based treatments for certain pathological behavior, and raised concerns about the use and misuse of scientific evidence. "The mere fact that brain processes give rise to the behavior isn't enough to excuse it," said Farah. "But the law does recognize some psychological conditions that diminish responsibility, and if neuroscience knows something about the neural processes underlying these conditions, it can aid in their diagnoses." Among the critical issues: how new insights about brain maturity should be used when assessing teenage crimes. "There currently is debate about whether adolescent brain maturity should be considered in sentencing for a crime or help determine what would be the proper deterrent for future adverse behavior," explained Giedd. "...What's difficult is applying this to individuals, as there are so many exceptions to the rule - there are many mature teens, and likewise immature people in their twenties and thirties. So the real challenge is going from group averages to individual prediction or characterization."
Another issue is how a deeper understanding of addiction should affect sentencing. Said Leshner, "By combining the health approach to the criminal justice approach, I think you have a better chance of having effective and acceptable public policy. If you don't deal with the illness or the brain part of drug addiction, you have much less chance of actually reducing the behavior you don't like, whether it's drug using or committing crimes."
More information: For the complete discussion with Alan Leshner, Martha Farah and Jay Giedd, visit: http://www.kavlifo … -brain-trial
Provided by The Kavli Foundation
"The brain on trial." December 12th, 2011. http://medicalxpress.com/news/2011-12-brain-trial.html
Posted by
Robert Karl Stonjek

Amazing Train Routes









Why do people defend unjust, inept, and corrupt systems?




Why do we stick up for a system or institution we live in—a government, company, or marriage—even when anyone else can see it is failing miserably? Why do we resist change even when the system is corrupt or unjust? A new article in Current Directions in Psychological Science, a journal published by the Association for Psychological Science, illuminates the conditions under which we're motivated to defend the status quo—a process called "system justification."
System justification isn't the same as acquiescence, explains Aaron C. Kay, a psychologist at Duke University's Fuqua School of Business and the Department of Psychology & Neuroscience, who co-authored the paper with University of Waterloo graduate student Justin Friesen. "It's pro-active. When someone comes to justify the status quo, they also come to see it as what should be."
Reviewing laboratory and cross-national studies, the paper illuminates four situations that foster system justification: system threat, system dependence, system inescapability, and low personal control.
When we're threatened we defend ourselves—and our systems. Before 9/11, for instance, President George W. Bush was sinking in the polls. But as soon as the planes hit the World Trade Center, the president's approval ratings soared. So did support for Congress and the police. During Hurricane Katrina, America witnessed FEMA's spectacular failure to rescue the hurricane's victims. Yet many people blamed those victims for their fate rather than admitting the agency flunked and supporting ideas for fixing it. In times of crisis, say the authors, we want to believe the system works.
We also defend systems we rely on. In one experiment, students made to feel dependent on their university defended a school funding policy—but disapproved of the same policy if it came from the government, which they didn't perceive as affecting them closely. However, if they felt dependent on the government, they liked the policy originating from it, but not from the school.
When we feel we can't escape a system, we adapt. That includes feeling okay about things we might otherwise consider undesirable. The authors note one study in which participants were told that men's salaries in their country are 20% higher than women's. Rather than implicate an unfair system, those who felt they couldn't emigrate chalked up the wage gap to innate differences between the sexes. "You'd think that when people are stuck with a system, they'd want to change it more," says Kay. But in fact, the more stuck they are, the more likely are they to explain away its shortcomings. Finally, a related phenomenon: The less control people feel over their own lives, the more they endorse systems and leaders that offer a sense of order.
The research on system justification can enlighten those who are frustrated when people don't rise up in what would seem their own best interests. Says Kay: "If you want to understand how to get social change to happen, you need to understand the conditions that make people resist change and what makes them open to acknowledging that change might be a necessity."
Provided by Association for Psychological Science
"Why do people defend unjust, inept, and corrupt systems?." December 12th, 2011. http://medicalxpress.com/news/2011-12-people-defend-unjust-inept-corrupt.html
Posted by
Robert Karl Stonjek

A Captivating Scene


Rama breaking Shiva's bow“One is looking at the arena and the city with amazement. Their mind and eyes are so attracted to that place that they cannot even blink.” (Janaki Mangala, 12)
ranga bhūmi pura kautuka eka nihārahiṃ |
lalaki subhāhiṃ nayana mana pheri na pāvahiṃ ||
Goswami Tulsidas here continues his description of the scene of the svayamvara held for Janaki, the daughter of King Janaka, many thousands of years ago. The city itself was a wonderful sight, and the rangabhumi, or arena holding the event’s main festivities, was also something to behold. People from far and wide arrived for this event, and they were not disappointed by what they first saw. Indeed, their reaction was one full of so much astonishment that their minds and eyes were fixed upon the main attraction. Many couldn’t even bring themselves to blink, for they didn’t want to deprive their eyes of the splendid vision for a second. Based on what would happen that day and who else would arrive later on, that elation was set to only increase.
If you travel from a distant place to attend an event, when you first see the arena or place where the event will take place, you will likely feel some happiness. “Ah, I have travelled so far, and look! There is the place where we are going. See how beautiful it looks. Let’s hurry up and park the car so that we can get a better view.” From the initial sighting the anticipation increases, and with anticipation comes an enhanced feeling of excitement when the event actually takes place.
Shiva's bowJanaki’s svayamvara wasn’t a rock concert, the performance of a dramatic play, or even the forum for a grand speech. Rather, the rangabhumi in question was holding a contest, one requiring immense strength. There was a famous bow belonging to Lord Shiva that was so heavy that even heavenly figures couldn’t dream of moving it. The contest was simple: lift the bow to win Janaka’s daughter’s hand in marriage.
Why would so many people travel to Janaka’s city? Janaki was the most beloved princess, endowed with every virtuous quality. Obtaining a good wife is akin to coming in to a large fortune, but not necessarily as we’d initially imagine it. When you win the lottery or strike oil, you hope to be financially secure for the rest of your life. As woman is the energy of man, obtaining a good wife means that you will have support in your journey through life, that you will have someone to correct you when you do something wrong and lift you up when you don’t feel up to performing your occupational duties.
The Vedic tradition reveals that the spirit soul is the essence of identity, and when coupled with a material covering, the aim in life becomes the forging of a permanent God consciousness. As this is not the natural inclination of a living entity who is born ignorant and required to undergo extensive education, rules, regulations and purificatory rites are instituted which span from the time of birth all the way up until death. Knowing which duties to perform and when is quite difficult; hence expert guidance from spiritual leaders is required.
In the middle stages of life, one can enter marriage to get support from a life partner, someone to live with you day in and day out. In addition, through marriage the natural urges for sex life can be acted upon in a regulated manner, thereby ensuring that society is filled with wanted children who are raised properly. From the protection of women through marriage, so many benefits come.
Sita DeviJanaki was no ordinary woman. She was found while as a baby in the ground that Janaka planned to plough for a religious sacrifice. He was childless at the time, and since he harbored such great affection for her, he took her in as his daughter. He didn’t run the risk of taking someone else’s baby because at the time of finding her, a voice in the sky came upon the scene and told Janaka that this girl belonged to him in all righteousness, or dharma.
Janaka lived by dharma, so he knew that when Sita reached an appropriate age, he had to find a husband for her. Not knowing her family ancestry and considering her tremendous virtues, Janaka decided to hold a contest and invite kings from around the world. If no one could lift Lord Shiva’s bow it would be a sign from above that no man was worthy of having Sita for a wife.
Everything about Janaka was first class, including his hospitality. The excitement over the svayamvara was well worth it. The visitors travelling with their family and royal entourages were not disappointed by what they saw in Tirahuta, Janaka’s capital city. People looked at the sacrificial arena in amazement, so much so that they couldn’t believe what they were seeing. As if Janaka knew the event would be talked about for thousands of years into the future, he made sure not to skimp on pomp. The king who had the greatest wealth in the form of his daughter made sure not to be frugal with regards to her marriage ceremony.
Sita DeviWhat Janaka didn’t initially know was that his daughter was the goddess of fortune herself, Lakshmi Devi. Think of the Supreme Lord’s most confidential associate, someone who gives Him the most pleasure. That person is Lakshmi, who has many different expansions and forms in the spiritual world. To coincide with her husband’s descent to earth as the hero of the Raghu dynasty, Lord Rama, Lakshmi appeared as Sita. She specifically chose Janaka as a father because of his qualifications. He had not a hint of sin in him, and he had the purity required for offering affection to the goddess of fortune.
Janaka did not want riches, but he didn’t shun them either. Whatever was needed to abide by dharma, Janaka would do. As an expert transcendentalist, he was above attraction and aversion, and yet he harbored immense affection for Sita. To prove that this love wasn’t of the material variety, Sita would bring to Janaka the Supreme Lord Himself as a son-in-law. Normally attraction and aversion are considered detrimental because they are based on maya, or illusion. If I’m walking down the road on a hot day and I think I see a pool of water up ahead, I might get excited. The attraction for the water will keep me running faster towards the destination spot. When I get there, however, I see that the water was a mirage, just a bunch of heat rays rising up from the surface of the ground. In this case both the initial excitement and ending dejection were not wise, for they were based on illusion.
The material body is like a bubble, which gets created at some point and is then quickly destroyed. Even if the body remains manifest for one hundred years, in the grand scheme that is an insignificant amount of time. Though the material forms do exist, since their duration of existence is so short, they can be considered illusory. Harboring attachment to these forms is detrimental because it keeps one in the dark about their real identity as eternal spirit. Having aversion to these forms based on the same mindset is also detrimental because since these forms are temporary, what is the use in hating them?
“Even kings like Janaka and others attained the perfectional stage by performance of prescribed duties. Therefore, just for the sake of educating the people in general, you should perform your work.” (Lord Krishna, Bhagavad-gita, 3.20)
King Janaka watching daughter SitaBoth attachment and aversion can be purified when they are used to further one’s real position as servant of God. With Janaka, his initial aversion to material life kept him on the straightened path. He was Brahman realized, so he knew that the spirit soul is the essence of identity within all forms and that to act out of obligation to uphold righteousness is the right way to behave. He did what was prescribed for his order, not caring for the results of action either way.
At the same time, Janaka was not bereft of attachment. His love for Sita could not be measured, and that same love would be harbored for Shri Rama and His younger brother Lakshmana. The first time Janaka saw the two brothers, he thought that maybe they were embodiments of the Brahman he had meditated on for so long. The Lord’s direct potency of yogamaya helped Janaka’s transcendental attachment increase. From transcendental love Janaka was able to experience more pleasure and receive benefits not granted to anyone else. Since Janaka had Sita as a daughter and Rama as a son-in-law, we can say that there was never a king like him in the past and there will never be one like him in the future.
The svayamvara ceremony inherited that uniqueness. Those staring in wonder were feasting on the fruit of their eyes. Though the eyes are composed of material elements, when they are used to further one’s God consciousness, they take on their true value. The attendees of the svayamvara got to see the city of Janakpur, which is like a tilaka, or sacred mark, on this earth. Sita Devi appeared in that great land and her marriage to Rama took place there as well. For the travellers, so many spiritual merits were accumulated just by going there. Through his piety, Janaka would attract many people to a ceremony that would benefit them immensely.
That great kings’ good character continues to generously benefit people today, those who are fortunate enough to mentally travel back to that famous day when Rama lifted Lord Shiva’s bow and won Sita’s hand in marriage. The ears are meant for hearing about God, and especially His name. At Sita’s svayamvara everyone would see Sita and Rama and recite their names. Thus even the many princes who failed to lift Lord Shiva’s bow got the victory of witnessing Sita and Rama married in an extravagant ceremony, one that you couldn’t take your eyes off of.
In Closing:
Stare at something long and get attachment,
To possess cherished item life’s attainment.
Visitors to Janaka’s ceremony this way felt,
From vision of bow and arena hearts did melt.
Finding baby Sita Janaka was charmed,
Attachment to her piety not harmed.
Rather love for her brought God to him,
When Rama bow’s contest did win.
Even failed suitors were there to witness,
Union of Sita and Rama, eyes to bless.

Researchers illuminate the gap between experience and association




(Medical Xpress) -- In the moments after lightning streaks through the sky, we wait for the clap of thunder that experience has told us is likely to follow. In a finding that may have implications for treating Alzheimer’s disease, researchers at the RIKEN-MIT Center for Neural Circuit Genetics in the Picower Institute for Learning and Memory at MIT report in the Dec. 9 issue of Science that they have identified for the first time the part of the brain responsible for that delayed association.
The entorhinal cortex, or EC, is one of the first brain areas affected in Alzheimer’s disease. Interestingly, early onset Alzheimer’s affects performance in memory tasks with a delay between learning and recalling items. “Our findings provide new insights into how patients with Alzheimer’s disease develop deficits in working memory with consequent failure of the formation of episodic memory,” said study co-author Junghyup Suh, research scientist at the Picower Institute. “ In addition, by identifying the circuits responsible for the cognitive deficits in human patients with the disease, we begin to lay a potential framework for selective therapeutic intervention in Alzheimer’s disease.”
Anticipating thunder after seeing lightning or a bee sting after hearing a buzzing insect is called temporal association because the first sensory experience is separated from its associated experience by a short gap in time.
RIKEN-MIT Center researchers found that inputs from a part of the brain called the EC layer III to the hippocampus, the seahorse-shaped part of the brain responsible for memory formation and retrieval, are crucial for temporal associations. “This study shows for the first time that the EC is important for processing non-spatial information such as a time element of episodic memory,” Suh said. The research was conducted in the laboratory of Susumu Tonegawa, Picower Professor of Biology and Neuroscience at the Picower Institute. In addition to Suh, study authors include Picower Institute postdoctoral associate Alexander J. Rivest; Toshiaki Nakashiba, research scientist; Takashi Tominaga of Tokushima Bunri University in Japan; and Susumu Tonegawa, Picower Professor of Neuroscience at the Picower Institute.
The EC acts as an interface between the hippocampus and the neocortex. The EC is thought to play a role in retrieving and consolidating spatial memories and in fine-tuning place cells in the hippocampus. Previous research outside of MIT had found that different kinds of cells in the EC are tied to where an animal is in space, suggesting the EC relays spatial information to the hippocampus. In addition, studies have shown that certain cells in the EC can fire continuously for tens of seconds; this type of persistent firing can help maintain sensory information (such as buzzing and lightning) through time in absence of sensory inputs.
“This study is among the first to examine the interactions between the hippocampus and its adjacent cortical areas in cognitive processes using genetic tools with great temporal and spatial specificity,” Suh said. “It also opens the door to future research with regard to how the hippocampus and EC communicate, process information and guide behaviors.”
Provided by Massachusetts Institute of Technology
"Researchers illuminate the gap between experience and association." December 12th, 2011. http://medicalxpress.com/news/2011-12-illuminate-gap-association.html
Posted by
Robert Karl Stonjek

Neuroscientists visualize neural circuitry at once-unattainable depths



‘Mind-readers’ get a clearer view Figure 2: Visualization of fluorescently labeled neurons within a rectangular volume encompassing cells in the cerebral cortex and hippocampus. This image was captured with a specialized lens enabling penetration to a depth of 4 millimeters below the brain surface. Credit: 2011 Atsushi Miyawaki
A recent breakthrough in biological sample preparation by scientists at the RIKEN Brain Science Institute in Wako may give Astro Boy’s ‘x-ray vision’ a run for its money. By treating tissue samples with an easy-to-prepare mix of chemicals, Atsushi Miyawaki, Hiroshi Hama and their colleagues can render the brain and other tissues as clear as glass, a reversible transformation that gives researchers an unobstructed view of fluorescently labeled cells residing within1.
For decades, the limits of available technology have thwarted attempts to map the dizzying twists and turns of the brain. Neuroscientists have achieved some success in simpler organisms, such as the worm or fly, by using tiny blades to sequentially peel off ultrathin strips of tissue, which can then be imaged via electron microscopy and reassembled computationally. However, this approach is far too labor-intensive and time-consuming for the reconstruction of a system as complex as the mammalian nervous system.
More recent breakthroughs in optical microscopy technology and a rapidly growing arsenal of multi-colored fluorescent proteins have given researchers potent new tools for brain mapping. By restricting the expression of specific fluorescent labels to particular subsets of cells, one can clearly visualize neural circuits within their natural, three-dimensional context; however, the dense tissue of the brain tends to scatter light, limiting the depth to which such imaging strategies can penetrate.
Several research groups have developed ‘clearing agents’ that improve the transparency of biological samples, such as benzyl-alcohol/benzyl-benzoate (BABB) and a proprietary solution known as FocusClear, but each suffers important limitations. “BABB is an organic solvent that requires dehydration of samples to be cleared,” says Miyawaki. He adds that such treatment can greatly diminish overall sample fluorescence, “and FocusClear does not clear mouse brain samples [readily].” Accordingly, his team’s development of the reagent they call ‘Scale’ could open new frontiers in mouse brain imaging.
Going deeper
Scale initially emerged from the unexpected, chance observation that membranes composed of the material polyvinylidene fluoride, which normally resemble sheets of white paper, become completely transparent when soaked in a high-concentration urea solution. By tinkering with this solution, Miyawaki and colleagues arrived at ScaleA2, a mixture that achieves the same feat with biological tissues.
ScaleA2 can render a mouse brain essentially transparent within two weeks (Fig. 1). This treatment also causes the tissue to swell as a result of water uptake, but the researchers determined that specimens maintain their overall shape and proportions, suggesting that this expansion does not significantly affect the arrangement of the cellular structures being imaged.
In an initial test of their imaging approach, Miyawaki and colleagues found that cells within ScaleA2-treated samples fully retained their fluorescent labels, while tissues treated with BABB yielded greatly diminished signal. More importantly, the transparency induced by ScaleA2 allowed the researchers to visualize far deeper within the brain than before, even when using standard ‘one-photon’ microscopic approaches that are typically vulnerable to scattering and background image interference.
“Although the imaging depth limit of two-photon excitation fluorescence microscopy is usually around 0.7 millimeters in the brain, we were able to image fluorescent neurons with Scale down to a depth of 2 millimeters below the brain surface,” says Miyawaki. By designing a specialized microscope lens, they were able to penetrate still farther, to an unprecedented working distance of 4 millimeters below the brain surface (Fig. 2). The level of detail obtained with ScaleA2 proved sufficient for the researchers to map axonal connections between neurons in the corpus callosum, the bridge between the brain’s hemispheres, and also allowed them to analyze the interaction between neural stem cells and the vasculature within the developing mouse brain.
Since not all specimens are created equal, Miyawaki and colleagues also experimented with alternative Scale formulations for specialized imaging applications. One of these, ScaleU2, necessitates longer sample incubation, but results in less tissue expansion so may offer advantages for use with embryonic samples or other fragile tissues. In a preliminary experiment on 13.5-day-old mouse embryos, the researchers used ScaleU2 to visualize regions of active cell division in the diencephalon—a part of the forebrain.
Importantly, the effects of Scale treatment proved fully reversible, and samples that had recovered from clearing proved indistinguishable from their uncleared counterparts, reaffirming the minimal impact of this treatment on tissue structure.
‘Mind-readers’ get a clearer view Figure 1: After two weeks of treatment with ScaleA2, the mouse brain is transparent enough (left) to be readily traversed by the light from a laser beam (right).
Credit: 2011 H. Hama
et al.
A clear view of the future
Some researchers have designed especially ambitious strategies for neural circuit mapping, such as the ‘Brainbow’ mouse developed at Harvard Medical School, which combines large numbers of different fluorescent proteins to turn the mouse brain into a dazzling light show in which virtually every neuron stands out clearly from its neighbors. Miyawaki believes Scale should prove highly complementary to such efforts. “All the fluorescent proteins we’ve tested so far are resistant to high concentrations of urea, and should be usable,” he says, “and so this approach should be compatible with Brainbow.”
His team is already engaged in collaborations to apply Scale to targeted investigations in mice. Although the work described to date has focused on genetically expressed fluorescent markers, this approach should also be compatible with other labeling methodologies. Once such techniques have been developed, Scale should prove effective for working with larger tissue samples obtained from species that are not readily amenable to genetic modification, such as primates.
The biggest limitation seen by Miyawaki at present is the need to work with ‘dead’ tissue, but he suggests that even this may change. “Scale is currently limited to fixed biological samples,” he says, “but at some point in the future, there may be ‘live Scale’.”
Provided by RIKEN
"Neuroscientists visualize neural circuitry at once-unattainable depths." December 12th, 2011. http://medicalxpress.com/news/2011-12-neuroscientists-visualize-neural-circuitry-once-unattainable.html
Posted by
Robert Karl Stonjek

Tapping the brain orchestra



Tapping the brain orchestra This image shows a forest of neurons. Credit: Hermann Cuntz, modified by Klas Pettersen

Researchers at the Norwegian University of Life Sciences (UMB) and Forschungszentrum Julich in Germany have developed a new method for detailed analyses of electrical activity in the brain. The method, recently published in Neuron, can help doctors and researchers to better interpret brain cell signals. In turn, this may lead to considerable steps forward in terms of interpreting for example EEG measurements, making diagnoses and treatment of various brain illnesses.
Researchers and doctors have been measuring and interpreting electrical activity generated by brain cells since 1875. Doctors have over the years acquired considerable practical skills in relating signal shapes to different brain illnesses such as epilepsy. However, doctors have so far had little knowledge on how these signals are formed in the network of nerve cells.
"Based on methods from physics, mathematics and informatics, as well as computational power from the Stallo supercomputer in Tromsø, we have developed detailed mathematical models revealing the connection between nerve cell activity and the electrical signal recorded by an electrode," says Professor Gaute Einevoll at the Department of Mathematical Sciences and Technology (IMT) at UMB.
Microphone in a crowd
The problem of interpreting electrical signals measured by electrodes in the brain is similar to that of interpreting sound signals measures by a microphone in a crowd of people. Just like people sometimes all talk at once, nerve cells are also sending signals "on top of each other".
The electrode records the sounds from the whole orchestra of nerve cells surrounding it and there are numerous contributors. One cubic millimetre can contain as many as 100,000 nerve cells.
Treble and bass
Similar to bass and treble in a soundtrack, high and low frequency electrical signals are distinguished in the brain.
"This project has focused on the bass - the low frequency signals called "local field potential" or simply LFP. We have found that if nerve cells are babbling randomly on top of each other and out of sync, the electrode's reach is narrow so that it can only receive signals from nerve cells less than about 0.3 millimetres away. However, when nerve cells are speaking simultaneously and in sync, the range can be much wider," Einevoll says.
Large treatment potential
Better understanding of the electrical brain signals may directly influence diagnosing and treatment of illnesses such as epilepsy.
"Electrodes are already being used to measure brain cell activity related to seizures in epilepsy patients, as well as planning surgical procedures. In the future, LFP signals measured by implanted electrodes could detect an impending epilepsy seizure and stop it by injecting a suitable electrical current," Einevoll says.
"A similar technique is being used on many Parkinson's patients, who have had electrodes surgically implanted to prevent trembling," Researcher Klas Pettersen at UMB adds.
Einevoll and Pettersen also outline treatment of patients paralysed by spinal cord fracture as another potential area where the method can be used.
"When a patient is paralysed, nerve cells in the cerebral cortex continue to send out signals, but the signals do not reach the muscles, and the patient is thus unable to move arms or legs. By monitoring the right nerve cells and forwarding these signals to for example a robot arm, the patient may be able to steer by his or her thoughts alone," Einevoll says.
The Computational Neuroscience Group at UMB has already established contacts with clinical research groups in the USA and Europe for further research on using the approach in patient treatment.
More information: The research team recently published the article "Modeling the spatial reach of the LFP" in Neuron.
Provided by Norwegian University of Life Sciences
"Tapping the brain orchestra." December 12th, 2011. http://medicalxpress.com/news/2011-12-brain-orchestra.html
Posted by
Robert Karl Stonjek

Bangalore gets its Metro











Was Darwin wrong about emotions?




Contrary to what many psychological scientists think, people do not all have the same set of biologically "basic" emotions, and those emotions are not automatically expressed on the faces of those around us, according to the author of a new article published in Current Directions in Psychological Science, a journal published by the Association for Psychological Science. This means a recent move to train security workers to recognize "basic" emotions from expressions might be misguided.
"What I decided to do in this paper is remind readers of the evidence that runs contrary to the view that certain emotions are biologically basic, so that people scowl only when they're angry or pout only when they're sad," says Lisa Feldman Barrett of Northeastern University, the author of the new paper.
The commonly-held belief is that certain facial muscle movements (called expressions) evolved to express certain mental states and prepare the body to react in stereotyped ways to certain situations. For example, widening the eyes when you're scared might help you take in more information about the scene, while also signaling to the people around you that something dangerous is happening.
But Barrett (along with a minority of other scientists) thinks that expressions are not inborn emotional signals that are automatically expressed on the face. "When do you ever see somebody pout in sadness? When it's a symbol," she says. "Like in cartoons or very bad movies." People pout when they want to look sad, not necessarily when they actually feel sad, she says.
Some scientists have proposed that emotions regulate your physical response to a situation, but there's no evidence, for example, that a certain emotion usually produces the same physical changes each time it is experienced, Barrett says. "There's tremendous variety in what people do and what their bodies and faces do in anger or sadness or in fear," she says. People do a lot of things when they're angry. Sometimes they yell; sometimes they smile.
"Textbooks in introductory psychology says that there are about seven, plus or minus two, biologically basic emotions that have a designated expression that can be recognized by everybody in the world, and the evidence I review in this paper just doesn't support that view," she says. Instead of stating that all emotions fall into a few categories, and everyone expresses them the same way, Barrett says, psychologists should work on understanding how people vary in expressing their emotions.
This debate isn't purely academic. It has consequences for how clinicians are trained and also for the security industry. In recent years there's been an explosion of training programs that are meant to help security officers of all kinds identify people who are up to something nefarious. But this training might be misguided, Barrett says. "There's a lot of evidence that there is no signature for fear or anger or sadness that you could detect in another person. If you want to improve your accuracy in reading emotion in another person, you have to also take the context into account."
Incidentally, the theory that emotional expressions evolved for specific functions is normally attributed to Charles Darwin, in his book The Expression of the Emotions in Man and Animals. But Darwin didn't write that emotional expressions are functional. "If you're going to cite Darwin as evidence that you're right, you'd better cite him correctly," Barrett says. Darwin thought that emotional expressions – smiles, frowns, and so on –were akin to the vestigial tailbone – and occurred even though they are of no use.
Provided by Association for Psychological Science
"Was Darwin wrong about emotions?." December 13th, 2011. http://medicalxpress.com/news/2011-12-darwin-wrong-emotions.html
Posted by
Robert Karl Stonjek

The man with the golden brain



What’s the point of a brain? A fundamental question that has led Professor Daniel Wolpert to some remarkable conclusions about how and why the brain controls and predicts movement. In a recent talk for TED, Wolpert explores the research that resulted in him receiving the Golden Brain Award.
The sea squirt, a type of marine filter feeder, swims around looking for somewhere to settle down for the rest of its life. Once parked on a rock in a suitable spot it never moves again. So the first thing it does is eat its own brain. While this may seem a little rash to some, for Professor Daniel Wolpert it makes perfect evolutionary sense.
“To me it’s obvious that there’s no point in the brain processing or storing anything if it can’t have benefits for physical movement, because that’s the only way we improve our survival” says Wolpert. “I believe that to understand movement is to understand the whole brain. Memory, cognition, sensory processing – they are there for a reason, and that reason is action.”
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Wolpert is firmly convinced that movement is the underlying factor and final result behind every functional aspect of a brain. “There can be no evolutionary advantage to laying down memories of childhood, or perceiving the colour of a rose, if it doesn’t affect the way you’re going to move in later life” he says. A professor in the Department of Engineering, Wolpert examines computational models and uses simple behavioural experiments to describe and predict how the brain solves problems related to action. Through this combination of theoretical and behavioural work, Wolpert has begun to revolutionise the study of human sensorimotor control, the way in which the brain controls physical movement.
He was recently presented with the prestigious Golden Brain Award, from the California-based Minerva Foundation. The award is given to those producing original and outstanding research into the nature of the brain, regarded by many as the most complex object in the known universe.
So what occurs in the brain when humans produce movement? Science has long struggled with the mysteries of this question. Wolpert uses the example of the game of chess: “We have computers that can generate algorithms of possible chess moves at tremendous speeds, beating the best human chess players. But ask a machine to compete on a dextrous level, such as moving a chess piece from one square to another, and the most advanced robot will fail every time against the average five year old.”
The models employed by Wolpert and his team have yielded startling results, a possible glimpse into the patterns integral to our mental matrix. “It turns out the brain behaves in a very statistical manner, representing information about the world as probabilities and processes, which is possible to predict mathematically” says Wolpert. “We’ve shown that this is a very powerful framework for understanding the brain.”
For action to occur, a command is sent from the brain causing muscles to contract and the body to move. Sensory feedback is then received from vision, skin, muscles and so on, to help gauge success. Sounds simple, but a vast amount of misinformation or ‘noise’ is generated with even the most basic action, due to the imperfections in our senses and the almost incalculable variables of the physical world around us. “We work in a whole sensory/task soup of noise” says Wolpert. “The brain goes to a lot of effort to reduce the negative consequences of this noise and variability.”
The brain’s crystal ball
To combat this noise, our brains have developed a sophisticated predictive ability, so that every action is based on an orchestrated balance between current sensory data and, crucially, past experience. Memory is a key factor in allowing the brain to make the optimal ‘best guess’ for cutting through the noise, producing the most advantageous movement for the task. In this way, our brains are constantly attempting to predict the future.
“An intuitive example of this predictive ability might be returning a serve in tennis. You need to decide where the ball is going to bounce to produce the most effective return. The brain uses the sensory evidence, such as vision and sound, and combines it with experience, prior knowledge of where the ball has bounced in the past. This creates an area of ‘belief’, the brains best guess of where ball will hit court, and the command for action is generated accordingly.”
Movement can take a long time from command to muscles, which can leave us exposed. Like chess, we need to be anticipating several moves ahead, so the brain uses its predictive ability to try and internally replicate the response to an action as or even before it is made, a kind of inbuilt simulator. The brain then subtracts this simulation from our actual experience, so it isn’t adding to the noise of misinformation.
“For behavioural causality, we need to be more attuned to the outside world as opposed to inside our own bodies. When our neural simulator makes a prediction, it is only based on internal movement commands. The brain subtracts that prediction from the overall sensation, so that everything left over is hopefully external.”
But this can have intriguing effects on our perceptions of the physical world, and the consequences of our actions. “This is why we can’t tickle ourselves, as tickling relies on an inability to predict sensation, and your neural simulator has already subtracted the sensation from the signal” says Wolpert.
“But they hit me harder!”
A further example of this sensory subtraction occurred to Wolpert during a backseat bust-up between his daughters, a familiar experience for most parents during long car journeys. The traditional escalation of hostility was ensuing as each child claimed they got hit harder and so retaliated in kind.
Wolpert explains: “You underestimate a force when you generate it, so as one child hits another, they predict the sensory movement consequences and subtract it off, thinking they’ve hit the other less hard than they have. Whereas the recipient doesn’t make the prediction so feels the full blow. So if they retaliate with the same force, it will appear to the first child to have been escalated.”
This led to a simple but effective experiment being conducted called ‘tit for tat’, in which two adults sit opposite each other with their fingers on either side of a force transducer. They were asked to replicate the force demonstrated by each other when pushing against the others finger. Instead of remaining constant, a 70 percent escalation of force is recorded on each go. It seems that we really don’t know our own strength.
Deciding to act
The next challenge for Wolpert is investigating how we make the decision to act, and what happens in the brain if we change our minds after the initial decision. “We think that the fields of both decision making and action share a lot of common features, and our goal is to try and link them together to create a unifying model of how actions affect decisions and vice versa” says Wolpert.
“As we walk around the world, do our decisions depend on how much effort is required, and to what extend does perceived effort influence the decisions we make? Similarly, to what extent does perceived effort relate to the decision to change our minds? These are the questions we want to address.”
To this end, Wolpert is about to begin on a project for the Human Frontiers Science Program on linking decision to action. “We’ve developed robotic interfaces in the lab which allow us to control and create experiences that people won’t have had before.”
“We ask subjects to perform simple tasks using a joystick. Once they are in a rhythm, we generate forces that act proportionally to speed but perturb their arm in unusual ways, such as right angles, and see how they respond. This allows us to build a dataset on novel learning, how people adapt to various forces, and the decisions that they make in the process.”
Wolpert’s ultimate aim is to apply these models of the brain and how it controls movement to a greater understanding of brain disorders. “Five percent of the population suffers from diseases that affect movement. The hope is that we will not only understand what goes wrong in disease, but how to design better mechanisms for rehabilitation.”
Provided by University of Cambridge
"The man with the golden brain." December 13th, 2011. http://medicalxpress.com/news/2011-12-golden-brain.html
Posted by
Robert Karl Stonjek

Did a good sense of smell give us an evolutionary advantage over Neanderthals?



Did a good sense of smell give us an evolutionary advantage?Areas of the brain (Neanderthals on the left and modern humans on the right) that show differences in the sizes of temporal lobes (important for cognitive functions like language) and olfactory bulbs (for sense of smell).
(PhysOrg.com) -- Our sense of smell may have been as important as language in helping to give us, modern humans, an evolutionary advantage over other human relatives such as the Neanderthals, scientists report in the journal Nature Communications today.
Scientists have found that areas of the brain, the temporal lobes that correspond to cognition (for example language, memory and social function) and the olfactory bulbs that correspond to sense of smell, are larger in Homo sapiens compared to other human species. They are about 12% larger than those of Neanderthals, Homo neanderthalensis.
This suggests that these two areas work together and are more important in the evolution of the modern human brain than previously thought.
The research team was led by Markus Bastir and Antonio Rosas of the Spanish Natural Science Museum (CSIC) and included Chris Stringer and Robert Kruszynski at the Natural History Museum.
They analysed fossil skulls of hominins (ancient human relatives) including Homo sapiens, Homo neanderthalensis and Homo erectus. ‘We used a new and very precise way to measure and compare the volumes of areas inside hominin skulls dating up to nearly 2 million years ago,’ says Kruszynski.
They produced 3D models that helped reveal the detail of the internal structures. Kruszynski says, ‘Those of Homo sapiens - our own species - showed a surprising change of internal architecture compared with their predecessors, in the area housing the olfactory and temporal regions.
'Such changes were not so evident in the Neanderthal skulls that were studied. The different evolutionary pathways that these two species took may be part of the process that led to the distinct patterns found in Homo neanderthalensis and Homo sapiens.'
Until now, sense of smell has been thought of as less significant for humans compared to our other senses. Stringer explains, ‘It has been traditional to believe that we have reduced olfactory (smell) senses compared with other primates, and by implication, earlier humans. However, the data in this study suggest the opposite - that modern humans actually have an enhanced sense of smell.
‘This might be because of the greater range of environments in which we live and the greater range of foods modern humans exploit, and/or an increased social role for olfaction in our more complex social interactions.’
Links between smell and cognition
Scientists know that smells are processed in the same brain regions responsible for processing emotion, motivation, fear, memory, pleasure and attraction, making them an important aspect of social interactions. And olfaction is among the oldest sense in vertebrates, ‘the only one that establishes a direct connection between the brain and its environment,’ says Bastir.
The links between cognition and olfaction have caused neuroscientists to use the term ‘higher olfactory functions’ to describe those brain functions that combine the two.
The team says that the larger olfactory bulbs and temporal lobes of Homo sapiens would have made evolutionary sense in a social context. They would have contributed to kinship recognition, enhanced family relations, group cohesion and social learning, all crucial factors that scientists believe allowed modern humans to progress and become the only surviving human species.
More information: The paper 'Evolution of the base of the brain in highly encephalized human species' is published today in the journal Nature Communications. http://www.nature. … s/index.html
Provided by American Museum of Natural History
"Did a good sense of smell give us an evolutionary advantage over Neanderthals?." December 13th, 2011. http://www.physorg.com/news/2011-12-good-evolutionary-advantage-neanderthals.html
Posted by
Robert Karl Stonjek

Thinking The Worst


Ravana fighting off Jatayu“Is it possible that Sita - the princess of Videha who hails from Mithila and is the daughter of King Janaka - is together with that wretched Ravana after being forced by him? I think that maybe when the Rakshasa was taking away Sita, fearing Rama’s arrows he flew up so quickly that it caused her to fall.” (Hanuman, Valmiki Ramayana, Sundara Kand, 13.6-7)
kim nu sītā atha vaidehī maithilī janaka ātmajā ||
upatiṣṭheta vivaśā rāvaṇam duṣṭa cāriṇam |
kṣipram utpatato manye sītām ādāya rakṣasaḥ ||
bibhyato rāma bāṇānām antarā patitā bhavet |
Shri Hanuman, ever the sweetheart deserving victory in any endeavor he follows, here continues his mental review of the troublesome situation he found himself in. Assigned the herculean task of singlehandedly finding a missing princess inside of a city filled with the wickedest creatures of the world, Hanuman’s greatest difficulty came not from the opposing forces, but from his own mind, as his eagerness to meet the beloved wife of Lord Rama tested his patience. In reviewing what might have happened to the missing princess, Hanuman covered all possibilities, even those one should never think of.
Sita DeviWhat kind of thoughts should we never even entertain? With Sita Devi, the princess in question, the defining characteristic is devotion to Lord Rama. In fact, all of her other features are derivatives of this dedication, as her eyes dare not even rest on another man’s glance. Because of her spotless character, she was the object of desire for those consumed by their sense demands and her husband in turn the object of envy. That Lord Rama, the Supreme Lord descended to earth in a seemingly human form, would be envied is not surprising, for the very genesis of creation is rooted in this sentiment. Without a fervent desire to compete with the mightiest divine being, the original creator, the fountainhead of all energies, birth in a temporary land filled with miseries could not be possible.
Why would anyone be jealous of God? The real question is, “why wouldn’t everyone be?” The insecurities borne of ignorance keep us ever fearful of losing our possessions. If we see someone successful in business or life in general, it is natural for feelings of inferiority to arise. Only one who is self-satisfied, knowing the Truth and man’s position relative to the different energies emanating from Rama, will be non-envious. Such a person will rejoice over another’s success, and another’s sadness will be the cause of the greatest distress.
Knowing and loving God thus forms the primary goal for every form of life, but especially the human being, who is endowed with the ability to perfect consciousness. With proper adherence to austerity and religious practice from the time of birth, the mind can be taught to focus on activities which keep a steady target on the proper aim, one that is tied to the most pleasurable object. Rather than just a mental exercise devoid of pleasurable feelings, the highest form of religion known as bhakti-yoga, or devotional service, is meant to complement every practice with a corresponding level of joy and happiness. Among the gamut of emotions capable of emanating from the liberated spirit soul, the ability to praise and glorify others stands as the source of the highest pleasure. If a corresponding beneficiary is identified who has unlimited features and glories, the praising can continue uninterrupted and without motivation.
To see how bhakti is practiced perfectly, the Lord’s most intimate associates descend with Him from the spiritual world. When Lord Rama advented during the Treta Yuga, His eternal consort from the spiritual sky did so as well in the kingdom of Videha. Maharaja Janaka was the king at the time, and he was famous around the world for many reasons. He was extremely pious, chivalrous, charitable, and dedicated to dharma, or religiosity. A king who follows the prescribed duties assigned to his order meets unhappiness neither in this world nor in the next. Janaka was also known as Videha, which means “the bodiless”. He was so adept in mysticism that he had practically transcended the influence of every one of the senses acquired at the time of birth. He was self-realized and fully satisfied, thus he was deemed beyond emotion. It is much easier to abide by prescribed duties when carrying out your responsibilities doesn’t cause you any pain. When you are immune to both happiness and distress, following the righteous path is a piece of cake.
The fruit of Janaka’s religious practices would come on a fateful day when he was ploughing a field for the purpose of performing a grand sacrifice, or yajna. While ploughing, he found a small child emerging from the earth. Not knowing who she was, Janaka picked her up in his arms and held her with fatherly affection. Though he was Videha, he couldn’t help but be consumed with loving emotions upon holding the young girl. Then a voice from the sky, as if to alleviate Janaka’s concerns over who the child belonged to, told the king that the girl was his daughter in all righteousness. His concerns gone, Janaka took the child home with him and put her in the custody of his wife. Since she was born of the earth, he gave her the name Sita.
Sita holding lotus flowerThough she was a female and thus at the time not formally trained in Vedic principles and concepts, Sita still acquired the same attribute of dispassion found in her father. Born in a family known for its chivalry, righteousness, and detachment from sense gratification, Sita Devi was essentially a yogi in every respect. Of course the true source of her mystic abilities was her undying love and affection for the Supreme Lord, whom she would receive as a husband when she reached the appropriate age.
When Rama and Sita were residing in the forest of Dandaka, Ravana, the king of the Rakshasas living in Lanka, heard of Janaka’s daughter’s immense beauty. Despite having hundreds of the most beautiful princesses as wives, Ravana was intent on having Sita. As much as Janaka was videha, Ravana was that much attached to his senses, and then some. But Ravana knew he couldn’t fight Rama one on one and live to tell about it. Therefore he set up a ruse and quickly took Sita away while Rama wasn’t looking.
In the subsequent search for His wife, the all-knowing Rama enlisted the aid of a band of forest dwellers residing in Kishkindha. Hanuman was their most capable warrior, and through many trials and tribulations, he managed to make his way to the island of Lanka and then inside the inner apartments unseen. He searched and searched, for his devotion to Rama was impossible to properly measure. He had never seen Sita before, but that didn’t deter him in his mission. He was so anxious to meet her that he was willing to do pretty much anything to succeed.
After seeing so many beautiful women and unearthing practically every inch of the island, Hanuman stopped and reflected for a moment. It’s completely understandable that his enthusiasm would be tempered every so often by doubts and fears. Hanuman was not worried about his own well-being. He was concerned about the other members of the monkey-army, Sugriva [the leader of the monkeys], and Rama and His younger brother Lakshmana. They were all counting on him, so Hanuman did not want to let them down.
Shri HanumanIn the above referenced verse from the Ramayana, Hanuman is running through the possibilities of what might have happened. We see that he briefly contemplated whether Sita might have agreed to Ravana’s advances. But to rightly convince himself otherwise, Hanuman remembered that Sita was brought up in the line of Videha kings; therefore she could never be tempted by anyone’s advances, no matter how beautiful or powerful the man was. She loved Rama for who He was, not because of any of His outward features, though the Lord wasn’t lacking any opulence. Sita is a pure yogi, so she knows that the soul is meant to be attached to the Supreme Soul, the plenary expansion of God residing within the hearts of all living entities.
Abandoning that incorrect line of thinking, Hanuman next thinks that maybe Sita fell down while Ravana was flying away in haste. The demon would obviously be afraid of Rama’s arrows coming his way during the escape. Prior to Ravana’s taking of Sita, Rama had dispatched 14,000 of Ravana’s Rakshasa associates to the land of Yamaraja, the god of justice. How one man can kill that many fighters by himself is known only to the Supreme Lord and those ever devoted to Him. Ravana was no fool in this regard. He was thus surely in a hurry to get out of Dandaka and back to his island of Lanka, which was strategically situated far away from any mainland.
Hanuman would eventually fight on, pushing aside his fears and concerns. What else could he do? Give up? That would serve no purpose. The perseverance shown in devotional service brings the highest reward, for it validates the worthiness of the mission of life to the dedicated worker. Hanuman didn’t require this validation, but through his concerns his love for Rama only increased. They say familiarity breeds contempt, but with Hanuman the more he thinks of his beloved Sita and Rama, the more he becomes attached to them. Similarly, anyone fortunate enough to follow Hanuman’s travels through Lanka and his eventual finding of Sita will only learn to love him more and more with each passing day. Having his association is the greatest boon, as Hanuman carefully plants the seed of love for the Supreme Lord within the heart of the sincere devotee. By carefully watering that seed with regular chanting of the holy names, “Hare Krishna Hare Krishna, Krishna Krishna, Hare Hare, Hare Rama Hare Rama, Rama Rama, Hare Hare”, the effects of the senses become mitigated, and the bodiless sincere soul never strays from the purified platform of God consciousness.
Every party involved kept hope alive. Sita remained within her body by always thinking of the glories of her husband. This unique practice is a lesson for every soul suffering from the pangs of material existence. Even Hanuman followed this practice when he met difficulty. And why should there not be bumps along the road of life? For the fruit of our existence to be acquired and tasted, it must be something we really want. If devotion to the Lord is just handed to us, the fruit might be rejected or held off to be enjoyed later on. On the other hand, one who becomes hungrier and hungrier for finding that transcendental taste will relish the devotion instilled within their heart through solid practice of bhakti.
Hanuman chantingAnyone who finds themselves in distressful situations can follow the path laid down by Sita and Hanuman. In fact, if we concentrate our minds on their remembrance of Rama, their constant hope of being reunited with Him and seeing a smile on His most beautiful face, there is every chance at succeeding. Sita and Hanuman humble even the proudest person through their practically impossible to believe love for the Lord of the universe. Their pastimes and characteristics would not be believable were it not for the visual evidence that is the exhibition of divine love by the sadhus following in their line. The devotees prove that God exists and that He is most satisfied by love and devotion. Shri Rama doesn’t necessarily reside in the ashrama of the yogi, in the home of an opulent king, or in a place of sacrifice. He stays wherever His name, fame and glories are constantly recited. He resides with those who make the heart the resting place for these transcendental features.
In Closing:
“Is it possible for Sita of Videha’s line,
To give in to the Rakshasa of the worst kind?
Perhaps Ravana in a rush to retreat,
Rama’s arrows perform amazing feats.
Flying in a rush to avoid arrows abound,
Perhaps Sita then fell from chariot to ground.”
Hanuman knew that these thoughts not true,
But Sita not found, what else could he do?
Thought the worst but victory he’d see,
Without love for Rama he cannot be.

Good Ones!!!

1 stone is enough to break a glass.
1 sentence is enough to break a heart.
1 sec is enough to fall in love.

But y d hell 1chapter is not enough to pass in exam...???


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