by Biomechanism

Sugar, cholesterol, phosphates, zinc – a healthy body is amazingly good at keeping such vital nutrients at appropriate levels within its cells.

From an engineering point of view, one all-purpose model of the pump on the surface of a cell should suffice to keep these levels constant: When the concentration of a nutrient, say, sugar, drops inside the cell, the pump mechanism could simply go into higher gear until the sugar levels are back to normal. Yet strangely enough, such cells let in their nutrients using two types of pump: One is active in “good times,” when a particular nutrient is abundant in the cell’s environment; the other is a “bad-times” pump that springs into action only when the nutrient becomes scarce. Why does the cell need this dual mechanism?

A new Weizmann Institute study, reported in Science, might provide the answer. The research was conducted in the lab of Prof. Naama Barkai of the Molecular Genetics Department by postdoctoral fellow Dr. Sagi Levy and graduate student Moshe Kafri with lab technician Miri Carmi.

It had been known for a while that when the levels of phosphate or zinc drop in the surroundings of a yeast cell, the number of “bad-times” pumps on the cell surface soars up to a hundred-fold. When phosphate or zinc becomes abundant again, the “bad-times” pumps withdraw while the “good-times” pumps return to the cell surface in large numbers.

In their new study, the scientists discovered that cells that repress their “bad-times” pumps when a nutrient is abundant were much more efficient at preparing for starvation and recovering afterwards than the cells genetically engineered to avoid this repression. The conclusion: The “good-times” pumps apparently serve as a signalling mechanism that warns the yeast cell of approaching starvation. Such advance warning gives the cell more time to store up on the scarce nutrient; the thorough preparation also helps the cell grow faster once starvation is over.

Thus, the dual-pump system is part of a regulatory mechanism that allows the cell to deal effectively with fluctuations in nutrient supply. This clever mechanism offers cell survival advantages that only one type of pump could not provide.

If these findings apply to human cells, they could explain how our bodies maintain adequate levels of various nutrients in tissues and organs. Understanding the dual-pump regulation could be crucial because it might be defective in various metabolic disorders.

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Prof. Naama Barkai’s research is supported by the Helen and Martin Kimmel Award for Innovative Investigation; the Jeanne and Joseph Nissim Foundation for Life Sciences Research; the Carolito Stiftung; Lorna Greenberg Scherzer, Canada; the estate of John Hunter; the Minna James Heineman Stiftung; the European Research Council; and the estate of Hilda Jacoby-Schaerf. Prof. Barkai is the incumbent of the Lorna Greenberg Scherzer Professorial Chair.

Courtesy Weizmann Institute of Science in Rehovot, Israel