Why Do Cells Age? Discovery of Extremely Long-Lived Proteins May Provide Insight Into Cell Aging and Neurodegenerative Diseases

This microscope image shows extremely long-lived proteins, or ELLPs, glowing green on the outside of the nucleus of a rat brain cell. DNA inside the nucleus is pictured in blue. The Salk scientists discovered that the ELLPs, which form channels through the wall of the nucleus, lasted for more than a year without being replaced. Deterioration of these proteins may allow toxins to enter the nucleus, resulting in cellular aging. (Credit: Courtesy of Brandon Toyama, Salk Institute for Biological Studies)

Science Daily  — One of the big mysteries in biology is why cells age. Now scientists at the Salk Institute for Biological Studies report that they have discovered a weakness in a component of brain cells that may explain how the aging process occurs in the brain.

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The scientists discovered that certain proteins, called extremely long-lived proteins (ELLPs), which are found on the surface of the nucleus of neurons, have a remarkably long lifespan.

While the lifespan of most proteins totals two days or less, the Salk Institute researchers identified ELLPs in the rat brain that were as old as the organism, a finding they reported February 3 in Science.

The Salk scientists are the first to discover an essential intracellular machine whose components include proteins of this age. Their results suggest the proteins last an entire lifetime, without being replaced.

ELLPs make up the transport channels on the surface of the nucleus; gates that control what materials enter and exit. Their long lifespan might be an advantage if not for the wear-and-tear that these proteins experience over time. Unlike other proteins in the body, ELLPs are not replaced when they incur aberrant chemical modifications and other damage.

Damage to the ELLPs weakens the ability of the three-dimensional transport channels that are composed of these proteins to safeguard the cell's nucleus from toxins, says Martin Hetzer, a professor in Salk's Molecular and Cell Biology Laboratory, who headed the research. These toxins may alter the cell's DNA and thereby the activity of genes, resulting in cellular aging.

Funded by the Ellison Medical Foundation and the Glenn Foundation for Medical Research, Hetzer's research group is the only lab in the world that is investigating the role of these transport channels, called the nuclear pore complex (NPC), in the aging process.

Previous studies have revealed that alterations in gene expression underlie the aging process. But, until the Hetzer lab's discovery that mammals' NPCs possess an Achilles' heel that allows DNA-damaging toxins to enter the nucleus, the scientific community has had few solid clues about how these gene alterations occur.

"The fundamental defining feature of aging is an overall decline in the functional capacity of various organs such as the heart and the brain," says Hetzer. "This decline results from deterioration of the homeostasis, or internal stability, within the constituent cells of those organs. Recent research in several laboratories has linked breakdown of protein homeostasis to declining cell function."

The results that Hetzer and his team just report suggest that declining neuron function may originate in ELLPs that deteriorate as a result of damage over time.

"Most cells, but not neurons, combat functional deterioration of their protein components through the process of protein turnover, in which the potentially impaired parts of the proteins are replaced with new functional copies," says Hetzer.

"Our results also suggest that nuclear pore deterioration might be a general aging mechanism leading to age-related defects in nuclear function, such as the loss of youthful gene expression programs," he adds.

The findings may prove relevant to understanding the molecular origins of aging and such neurodegenerative disorders as Alzheimer's disease and Parkinson's disease.

In previous studies, Hetzer and his team discovered large filaments in the nuclei of neurons of old mice and rats, whose origins they traced to the cytoplasm. Such filaments have been linked to various neurological disorders including Parkinson's disease. Whether the misplaced molecules are a cause, or a result, of the disease has not yet been determined.

Also in previous studies, Hetzer and his team documented age-dependent declines in the functioning of NPCs in the neurons of healthy aging rats, which are laboratory models of human biology.

Hetzer's team includes his colleagues at the Salk Institute as well as John Yates III, a professor in the Department of Chemical Physiology of The Scripps Research Institute.

When Hetzer decided three years ago to investigate whether the NPC plays a role in initiating or contributing to the onset of aging and certain neurodegenerative diseases, some members of the scientific community warned him that such a study was too bold and would be difficult and expensive to conduct. But Hetzer was determined despite the warnings.

Warning! Collision imminent! The brain's quick interceptions help you navigate the world

Researchers at The Neuro and the University of Maryland have figured out the mathematical calculations that specific neurons employ in order to inform us of our distance from an object and the 3-D velocities of moving objects and surfaces relative to ourselves.

When you are about to collide into something and manage to swerve away just in the nick of time, what exactly is happening in your brain? A new study from the Montreal Neurological Institute and Hospital – The Neuro, McGill University shows how the brain processes visual information to figure out when something is moving towards you or when you are about to head into a collision. The study, published in the Proceedings of the National Academy of Sciences (PNAS), provides vital insight into our sense of vision and a greater understanding of the brain.

Researchers at The Neuro and the University of Maryland have figured out the mathematical calculations that specific neurons employ in order to inform us of our distance from an object and the 3D velocities of moving objects and surfaces relative to ourselves. Highly specialized neurons located in the brain's visual cortex, in an area known as MST, respond selectively to motion patterns such as expansion, rotation, and deformation. However, the computations underlying such selectivity were unknown until now.

Using mathematical models and sophisticated recording techniques, researchers have discovered how individual MST neurons function. "Area MST is typical of high-level visual cortex, in that information about important aspects of vision can be seen in the firing patterns of single neurons. A classic example is a neuron that only fires when the subject is looking at the image of a particular face. This type of neuron has to gather information from other neurons that are selective to simpler features, like lines, colors, and textures, and combine these pieces of information in a fairly sophisticated way," says Dr. Christopher Pack, neuroscientist at The Neuro and senior author. "Similarly, for motion detection, neurons have to combine input from many other neurons earlier in the visual pathway, in order to determine whether something is moving toward you or just drifting past." The brain's visual pathway is made up of building blocks. For example, neurons in the retina respond to very simple stimuli, such as small spots of light. Further along the visual pathway, neurons respond to more complex stimulus such as straight lines, by combining inputs from neurons earlier on. Neurons further along respond to even more complex stimulus such as combinations of lines (angles), ultimately leading to neurons that can respond to, or recognize, faces and objects for example.

The research team found that a remarkably simple computation lies at the heart of this sophisticated neural selectivity: MST neurons appear to be capable of performing a multiplicative operation on their inputs. These inputs come from neurons one step earlier in the visual pathway, in a well-studied area known as MT. In other words, the inputs of MT neurons are multiplied in order to get the output of MST neurons. This turns out to be remarkably similar to what has been observed in other brain areas and in other species, suggesting it may reflect a general strategy by which brains process sensory information. "One interesting aspect of the computation is that it appears to be about the same as what other people have found in flies and beetles, suggesting that evolution solved this problem once, at least a few hundred million years ago."

"We developed a new motion stimulus with a morphing pattern flow (e.g. dots on a screen that are expansive, swirl around, circle to the right, contract etc) and recorded MST neurons responding to these stimuli," says Patrick Mineault, Ph.D. candidate at The Neuro and primary author on the study. "We circumvented the issue of increasing complexities of calculations along the various steps of the visual pathway by incorporating known data from neurons just one step earlier in the pathway - area MT, which precedes MST. As we now had measurements of the output of the MST neurons from the study's recordings, and already knew the input of MT neurons, we could calculate the math linking these two functions – and it turns out to be a multiplicative function." The mathematical models successfully account for the stimulus selectivity of some of the brain's complex motion neurons - which are vitally important in helping navigate us through the world.

Provided by McGill University

"Warning! Collision imminent! The brain's quick interceptions help you navigate the world." February 7th, 2012.http://medicalxpress.com/news/2012-02-collision-imminent-brain-quick-interceptions.html

 

Posted by
Robert Karl Stonjek

Engineers Weld Nanowires With Light

A titled, cross-sectional scanning electron microscope image of plasmonically welded nanowires of silver. (Credit: Mark Brongersma, Stanford)

Science Daily  — At the nano level, researchers at Stanford have discovered a new way to weld together meshes of tiny wires. Their work could lead to innovative electronics and solar applications. To succeed, they called upon plasmonics.

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One area of intensive research at the nanoscale is the creation of electrically conductive meshes made of metal nanowires. Promising exceptional electrical throughput, low cost and easy processing, engineers foresee a day when such meshes are common in new generations of touch-screens, video displays, light-emitting diodes and thin-film solar cells.

Standing in the way, however, is a major engineering hurdle: In processing, these delicate meshes must be heated or pressed to unite the crisscross pattern of nanowires that form the mesh, damaging them in the process.

In a paper just published in the journal Nature Materials, a team of engineers at Stanford has demonstrated a promising new nanowire welding technique that harnesses plasmonics to fuse the wires with a simple blast of light.

Self-limiting

At the heart of the technique is the physics of plasmonics, the interaction of light and metal in which the light flows across the surface of the metal in waves, like water on the beach.

"When two nanowires lay crisscrossed, we know that light will generate plasmon waves at the place where the two nanowires meet, creating a hot spot. The beauty is that the hot spots exist only when the nanowires touch, not after they have fused. The welding stops itself. It's self-limiting," explained Mark Brongersma, an associate professor of materials science engineering at Stanford and an expert in plasmonics. Brongersma is one of the study's senior authors.

"The rest of the wires and, just as importantly, the underlying material are unaffected," noted Michael McGehee, a materials engineer and also senior author of the paper. "This ability to heat with precision greatly increases the control, speed and energy efficiency of nanoscale welding."

In before-and-after electron-microscope images, individual nanowires are visually distinct prior to illumination. They lay atop one another, like fallen trees in the forest. When illuminated, the top nanowire acts like an antenna of sorts, directing the plasmon waves of light into the bottom wire and creating heat that welds the wires together. Post-illumination images show X-like nanowires lying flat against the substrate with fused joints.

Transparency

In addition to making it easier to produce stronger and better performing nanowire meshes, the researchers say that the new technique could open the possibility of mesh electrodes bound to flexible or transparent plastics and polymers.

To demonstrate the possibilities, they applied their mesh on Saran wrap. They sprayed a solution containing silver nanowires in suspension on the plastic and dried it. After illumination, what was left was an ultrathin layer of welded nanowires.

"Then we balled it up like a piece of paper. When we unfurled the wrap, it maintained its electrical properties," said co-author Yi Cui, an associate professor materials science and engineering. "And when you hold it up, it's virtually transparent."

This could lead to inexpensive window coatings that generate solar power while reducing glare for those inside, the researchers said.

"In previous welding techniques that used a hotplate, this would never have been possible," said lead author, Erik C. Garnett, PhD, a post-doctoral scholar in materials science who works with Brongersma, McGehee and Cui. "The Saran wrap would have melted far sooner than the silver, destroying the device instantly."

"There are many possible applications that would not even be possible in older annealing techniques," said Brongersma. "This opens some interesting, simple and large-area processing schemes for electronic devices -- solar, LEDs and touch-screen displays, especially."

This research was supported by the Center for Advanced Molecular Photovoltaics (CAMP) at Stanford University funded by King Abdullah University of Science and Technology (KAUST).

Exercise Triggers Stem Cells in Muscle

                   Science Daily — University of Illinois researchers determined that an adult stem cell in muscle is responsive to exercise, a discovery that may link exercise and muscle health. The findings could lead to new therapeutic techniques using these cells to rehabilitate injured muscle and prevent or restore muscle loss with age.

Mesenchymal stem cells (MSCs) in skeletal muscle have been known to be important for muscle repair in response to non-physiological injury, predominantly in response to chemical injections that significantly damage muscle tissue and induce inflammation. The researchers, led by kinesiology and community health professor Marni Boppart, investigated whether MSCs also responded to strain during exercise, and if so, how.

"Since exercise can induce some injury as part of the remodeling process following mechanical strain, we wondered if MSC accumulation was a natural response to exercise and whether these cells contributed to the beneficial regeneration and growth process that occurs post-exercise," said Boppart, who also is affiliated with the Beckman Institute for Advanced Science and Technology at the U. of I.

The researchers found that MSCs in muscle are very responsive to mechanical strain. They witnessed MSC accumulation in muscle of mice after vigorous exercise. Then, they determined that although MSCs don't directly contribute to building new muscle fibers, they release growth factors that spur other cells in muscle to fuse and generate new muscle, providing the cellular basis for enhanced muscle health following exercise.

A key element to the Illinois team's method was in exercising the mice before isolating the cells to trigger secretion of beneficial growth factors. Then, they dyed the cells with a fluorescent marker and injected them into other mice to see how MSCs coordinated with other muscle-building cells.

In addition to examining the cells in vivo, the researchers studied the cells' response to strain on different substrates. They found that MSC response is very sensitive to the mechanical environment, indicating that conditions of muscle strain affect the cells' activity.

"These findings are important because we've identified an adult stem cell in muscle that may provide the basis for muscle health with exercise and enhanced muscle healing with rehabilitation/movement therapy," Boppart said. "The fact that MSCs in muscle have the potential to release high concentrations of growth factor into the circulatory system during exercise also makes us wonder if they provide a critical link between enhanced whole-body health and participation in routine physical activity."

Next, the group hopes to determine whether these cells contribute to the decline in muscle mass over a person's lifetime. Preliminary data suggest MSCs become deficient in muscle with age. The team hopes to develop a combinatorial therapy that utilizes molecular and stem-cell-based strategies to prevent age-related muscle loss.

"Although exercise is the best strategy for preserving muscle as we age, some individuals are just not able to effectively engage in physical activity," Boppart said. "Disabilities can limit opportunities for muscle growth. We're working hard to understand how we can best utilize these cells effectively to preserve muscle mass in the face of atrophy."

The team published its findings in the journal PLoS One. The Illinois Regenerative Medicine Institute, the Ellison Medical Foundation and the Mary Jane Neer Foundation supported this work.

Why Bad Immunity Genes Survive: Study Implicates Arms Race Between Genes and Germs

This electron microscope image shows yellow particles of a mouse leukemia virus named Friend virus emerging or "budding" out of an infected white blood cell known as a T-cell. By allowing the Friend virus to mutate and evolve in mice, University of Utah researchers produced new evidence that an arms race between microbes and immune-system MHC genes is responsible for maintaining an amazing diversity of those genes, even though some of them are responsible for autoimmune and infectious diseases that make us sick. (Credit: Elizabeth Fischer and Kim Hasenkrug, NIH)      Science Daily  — University of Utah biologists have found new evidence for why mice, people and other vertebrate animals carry thousands of varieties of genes to make immune-system proteins named MHCs -- even though some of those genes make us susceptible to infections and to autoimmune diseases.

"Major histocompatibility complex" (MHC) proteins are found on the surface of most cells in vertebrate animals. They distinguish self from foreign, and trigger an immune response against foreign invaders. MHCs recognize invading germs, reject or accept transplanted organs and play a role in helping us smell compatible mates.

"This study explains why there are so many versions of the MHC genes, and why the ones that cause susceptibility to diseases are being maintained and not eliminated," says biology Professor Wayne Potts. "They are involved in a never-ending arms race that causes them, at any point in time, to be good against some infections but bad against other infections and autoimmune diseases."

By allowing a disease virus to evolve rapidly in mice, the study produced new experimental evidence for the arms race between genes and germs -- known technically as "antagonistic coevolution." The findings will be published online the week of Feb. 6, 2012, in the Proceedings of the National Academy of Sciences.

Potts, the senior author, ran the study with first author and former doctoral student Jason Kubinak, now a postdoctoral fellow in pathology. Other co-authors were biology doctoral student James Ruff, biology undergraduate C. Whitney Hyzer and Patricia Slev, a clinical assistant professor of pathology. The research was funded by the National Science Foundation and the National Institute of Allergy and Infectious Diseases.

Theories for the Diversity of Immune-System MHC Genes

Most genes in humans and other vertebrate have only one or two "alleles," which are varieties or variants of a single gene. Although any given person carries no more than 12 varieties of the six human MHC genes, the human population has anywhere from hundreds to 2,300 varieties of each of the six human genes that produce MHC proteins.

"The mystery is why there are so many different versions of the same [MHC] genes in the human population," Kubinak says, especially because many people carry MHCs that make them susceptible to many pathogens (including the AIDS virus, malaria and hepatitis B and C) and autoimmune diseases (including type I diabetes, rheumatoid arthritis, lupus, multiple sclerosis, irritable bowel disease and ankylosing spondylitis).

Scientists have proposed three theories for why so many MHC gene variants exist in vertebrate animal populations (invertebrates don't have MHCs), and say all three likely are involved in maintaining the tremendous diversity of MHCs:

-- An organism with more MHC varieties has a better immune response than organisms with fewer varieties, so over time, organisms with more MHCs are more likely to survive. However, this theory cannot explain the full extent of MHC diversity.

-- Previous research indicates people and other animals are attracted to the smell of potential mates with MHCs that are "foreign" rather than "self." Parents with different MHC variants produce children with more MHCs and thus stronger immune systems.

-- Antagonistic coevolution between an organism and its pathogens. Kubinak says: "We have an organism and the microbes that infect it. Microbes evolve to better exploit the organism, and the organism evolves better defenses to fight off the infection. One theory to explain this great diversity in MHC genes is that those competing interests over time favor retaining more diversity."

The Arms Race between Germs and MHC Genes

"You naturally keep genes that fight disease," Kubinak says. "They help you survive, so those MHC genes become more common in the population over time because the people who carry them live to have offspring."

Pathogens -- disease-causing viruses, bacteria or parasites -- infect animals, which defend themselves with MHCs that recognize the invader and trigger an immune response to destroy the invading pathogen.

But over time, some pathogens mutate and evolve to become less recognizable by the MHCs and thus evade an immune response. As a result, the pathogens thrive. MHCs that lose the battle to germs become less common because they now predispose people who carry them to get sick and maybe die. It was thought such disease-susceptibility MHC genes eventually should vanish from the population, but they usually don't.

Why? While some of those MHCs do go extinct, others can persist, for two reasons. First, some of the now-rare MHCs gain an advantage because they no longer are targeted by evolving microbes, so they regain an ability to detect and fight the same germ that earlier defeated them -- after that germ mutates yet again. Second, some of the rare MHCs can mount an effective immune response against completely different microbes.

How the Study was Performed; Implications of the Findings

The researchers studied 60 mice that were genetically identical, except the mice were divided into three groups, each with a different variety of MHC genes known as b, d and k, respectively.

A mouse leukemia virus named the Friend virus was grown in tissue culture and used to infect two mice from each of the three MHC types. The fast-evolving retrovirus grew within the mice for 12 days, attacking, enlarging and replicating within the spleen and liver. Virus particles in the spleen were collected, and the severity of illness was measured by weighing the enlarged spleen.

Then, virus taken from each of the first three pairs of mice (b, d and k) was used to infect another three pair of mice with the same MHC types. The process was repeated until 10 pairs of mice in each MHC type were infected, allowing the virus time to mutate.

In this first experiment, the biologists showed they could get the Friend virus to adapt to and thus evade the MHC variants (b, d or k) in the mouse cells it attacked.

Next, the researchers showed that the virus adapted only to specific MHC proteins. For example, viruses that adapted to and sickened mice with the MHC type b protein still were attacked effectively in mice that had the type d and k MHCs.

In the third experiment, the researchers showed that pathogen fitness (measured by the number of virus particles in the spleen) correlated with pathogen virulence (as measured by spleen enlargement and thus weight). So the virus that evaded MHC type b made mice with that MHC sicker.

Together, the experiments demonstrate "the first step in the antagonistic coevolutionary dance" between a virus and MHC genes, Potts says.

Potts says the findings have some important implications:

-- The use of antibiotics to boost productivity in dairy herds and other livestock is a major reason human diseases increasingly resist antibiotics. Selective breeding for more milk and beef has reduced genetic diversity in livestock, including their MHCs. So breeding more MHCs back into herds could enhance their resistance to disease and thus reduce the need for antibiotics.

-- Because their populations are diminished, endangered species have less genetic diversity, making them an easier target for germs. Potts says it would be desirable to breed protective MHCs back into endangered species to bolster their disease defenses.

-- Genetic variation of MHCs in people and other organisms is important for limiting the evolution and spread of emerging diseases. In effect, Potts and colleagues created emerging diseases by making a virus evolve in mice. "It's a model to identify what things change in viruses to make them more virulent and thus an emerging disease."

A therapist in your pocket

Brooding in your apartment on Saturday afternoon? A new smart phone intuits when you're depressed and will nudge you to call or go out with friends.

It's the future of therapy at a new Northwestern University Feinberg School of Medicine center where scientists are inventing web-based, mobile and virtual technologies to treat depression and other mood disorders. The phone and similar projects bypass traditional weekly therapy sessions for novel approaches that provide immediate support and access to a much larger population.

Also in the works at the National Institutes of Health-funded center: a virtual human therapist who will work with teens to prevent depression; a medicine bottle that reminds you to take antidepressant medication and tells your doctor if the dosage needs adjusting; a web-based social network to help cancer survivors relieve sadness and stress.

"We're inventing new ways technology can help people with mental health problems," said psychologist David Mohr, director of the new Center for Behavioral Intervention Technologies and a professor of preventive medicine at Northwestern's Feinberg School. "The potential to reduce or even prevent depression is enormous."

"These new approaches could offer fundamentally new treatment options to people who are unable to access traditional services or who are uncomfortable with standard psychotherapy," Mohr added. "They also can be offered at significantly lower costs, which makes them more viable in an era of limited resources."

The goal is for the center to become a national resource, offering a library of intervention technologies that will be available to other researchers.

Among the center's projects:

A REALLY SMART PHONE READS YOUR MOOD

A smart phone spots symptoms of depression by harnessing all the sensor data within the phone to interpret a person's location, activity level (via an accelerometer), social context and mood.

Are you making phone calls and getting e-mails, or are you home alone ruminating for hours? If the phone – which learns your usual patterns -- senses you are isolated, it will send you a suggestion to call or see friends. The technology, which still is being tweaked, is called Mobilyze! and has been tested in a small pilot study. It helped reduce symptoms of depression.

The new phone offers a powerful new level of support for people who have depression and intervenes to help them change their behavior in real time.

"By prompting people to increase behaviors that are pleasurable or rewarding, we believe that Mobilyze! will improve mood," Mohr said. "It creates a positive feedback loop. Someone is encouraged to see friends, then enjoys himself and wants to do it again. Ruminating alone at home has the opposite effect and causes a downward spiral."

YOU CAN'T FOOL THIS MEDICINE BOTTLE

A medicine bottle now being developed will track if you forgot your daily dose of antidepressant medication and remind you to take it. The savvy bottle addresses the common problem of patients who quickly stop taking antidepressant medications prescribed by their primary care doctors.

"People whose depression is being treated by primary care doctors often don't do very well, partly because patients don't take their medications and partly because the doctors don't follow up as frequently as they should to optimize the medication and dosage when necessary," Mohr said. "This pill dispenser addresses both issues."

The bottle is part of a MedLink system, which will include a mobile app that monitors the patient's depressive symptoms and any medication side effects and will provide tailored advice to manage problems. The information is then sent to the physician or health care provider with a recommendation, such as a change in the dose or drug, if necessary. The MedLink system also will be used to improve medication adherence in patients with schizophrenia and HIV.

VIRTUAL HUMAN COACHES TEENS IN SOCIAL SKILLS

A virtual programmable human will role play with adolescents and adults to teach social and assertiveness skills to prevent and treat depression. A prototype is being developed with researchers from the University of Southern California.

"We think this will be especially helpful for kids, who often are reluctant to see a therapist," Mohr said. The program will allow them to practice these behaviors in the safety of virtual space.

Existing online interventions for teens "look like homework," Mohr noted. The virtual human feels like a game, making it more likely to engage them.

The Northwestern lab will be evaluating a number of different types of social interactions that are hard for teens and adults.

"Having trouble with those situations makes people more vulnerable to depression," Mohr said. "When people have the confidence and skills to better manage difficult interpersonal interactions, they are less likely to become depressed." Previous research also has shown that intervening early in adolescents who have difficulty with social skills can help prevent the first onset of depression.

HELPING CANCER SURIVORS COPE WITH STRESS

Web-based content to help cancer survivors manage stress and depression is more effective when a human coach checks in on their progress via a phone call or e-mail.

"People are more likely to stick with an online program if they know that someone they like or respect can see what they're doing," Mohr said. His group is creating a closed social network and collaborative learning environment where peers can serve that function for each other.

"People can get feedback from the group, share goals and check in with members if someone has stayed offline for too long," Mohr said.

Provided by Northwestern University

"A therapist in your pocket." February 7th, 2012. http://medicalxpress.com/news/2012-02-therapist-pocket.html

 

Posted by
Robert Karl Stonjek

Resetting The Clock

 

“Always chanting My glories, endeavoring with great determination, bowing down before Me, these great souls perpetually worship Me with devotion.” (Lord Krishna, Bhagavad-gita, 9.14)

The sun is such a central component of life on earth that man revolves his routine around its relative position. The demarcation of a day is based on the full cycle of a rise and set by the sun, and with that day comes the routine of work, leisure, play, interaction, travel, rest, eating, etc. Yet man doesn’t have to do things this way. After waking up in the morning, he could just continue on from the previous day, as if no time had passed, as if the calendar hadn’t rolled onto another day. The routine, though, brings regulation, and regulation brings the ability to achieve a larger stated objective through a methodical process. When that same methodology is applied to fulfilling life’s ultimate mission, that of achieving the perfect consciousness while quitting the body, the rising and setting of the sun turn into welcomed and anticipated events.

Imagine seeing a giant housing structure full of intricacy. There are many floors and rooms, and the layout is such that you can’t begin to imagine how someone thought up the architecture. But someone did indeed envision the plan, which they subsequently put into place through the work of engineers and builders. As they say, “Rome wasn’t built in a day”, the project takes dedication and effort through many days, building little pieces here and there. In one way, forgetting about the big picture can be helpful, for by giving attention to tiny components that should fit perfectly well together, the mind doesn’t get overwhelmed about the length of the project. If someone were to tell us during youth that school would take twelve years to complete and we knew what that meant, we’d likely not want to attend.

Instead, we take one year at a time, focusing on advancing to the next grade. The same pattern is followed in any large scale project. In software application development, there are many complexities that need to be worked out. The enterprise application isn’t built in a day, but through building a solid codebase, adding routines, testing them, redesigning for efficiency and then retesting, eventually a solid program is completed.

According to the Vedas, the oldest scriptural tradition of the world, there is an Absolute Truth, an entity who is beyond duality. The world we live in is filled with polar opposites: heat and cold, light and darkness, success and failure, and birth and death. The Absolute Truth is the entity that is above these dichotomies. He is the same in birth and death, in heat and cold. The relative conditions don’t matter to Him because He is situated in complete knowledge and bliss. As His existence stretches the bounds of time and space, He is eternal.

The human form of body is meant for understanding that Absolute Truth. Indeed, the search for pleasure is rooted in the desire to associate with non-duality, though the feverish worker may not be aware of this fact. The bliss resulting from innovation in technology, from finishing a difficult project, or even from enjoying with friends and family is derived from the inherent relationship every living being has with the Absolute Truth.

The questions remain: how to connect with the Truth and where to find Him? How do we know that the Truth is a He? Doesn’t the masculine delineation make the Truth the opposite of something else, namely the feminine? If we have an opposite, isn’t that a duality? The Absolute Truth is referred to as a male because of its position of dominance. Mutually contradictory attributes must exist in the Supreme Person; otherwise the lack of features would indicate a defect. Man is looking for perfection in a worshipable figure, someone without defects. The daily stories in the news reveal this inner desire of man. A noted inventor, technologist, politician, athlete, or celebrity is propped up to “rock star” status and adored for their achievements. If they should fall, have a slip up, the same adoring media will punish them relentlessly, looking for any way possible to release their hatred.

The perfect being is known as God to most, but the Vedas provide many more names and descriptions for Him. Moreover, the Vedas say that the human brain cannot conceive of God on its own. Man must consult someone who knows the Truth from having both accepted the information from their own spiritual guide and from practicing the regulative principles of freedom. The highest form of religious practice is equated with freedom because through connection with the Absolute Truth, the duality of the phenomenal world ceases to be inhibiting. The sunlight shining bright in the eyes in the morning inhibits the ability to drive and to see what’s up ahead, but this doesn’t mean that the sunshine is bad. It all depends on how one uses the material elements. Through following the regulative principles of freedom, the material elements fulfill their proper purpose to the individual.

“One who can control his senses by practicing the regulated principles of freedom can obtain the complete mercy of the Lord and thus become free from all attachment and aversion.” (Lord Krishna, Bhagavad-gita

 

, 2.64)

And what purpose is that? Not surprisingly, it is to help the individual connect with God. The bona fide guru learned from his guru the principles of bhakti-yoga, or devotional service

 

. The guru’s chain of disciplic succession must originate with the Absolute Truth; otherwise the information presented will be based on mental speculation, which is flawed. The issue with following any regulative discipline is that the forces of material nature will operate all the same. If my goal is to lose weight, the enticements of sumptuous foods and late-night partying will get in the way of success. If my desire is to study for an important exam, then fatigue, inertia, and the allurements of relaxing without any pressures placed upon the mind can serve as wonderful distractions.

In bhakti practiced in a land conducive to illusion, the distractions are everywhere. The spirit soul has travelled through so many bodies in so many lifetimes that understanding the need for self-realization is rare enough. One who strives for understanding the position of the spirit soul, the essence of identity, is considered very fortunate. Once the sincere soul hears about the Absolute Truth and what’s required to connect with Him, they may accept the bhakti discipline in earnest, but the same past habits borne of attachment to use material elements for enjoyment in the absence of God’s association will still remain.

“After many births and deaths, he who is actually in knowledge surrenders unto Me, knowing Me to be the cause of all causes and all that is. Such a great soul is very rare.” (Lord Krishna

 

, Bg. 7.19)

In the pursuit for self-realization, for understanding the individual’s identity and how one is meant to be in God’s association, the repetition of days can be very helpful. In a life where one feels trapped, as if they are in a prison, the monotonous days can be too much to take. With the start of each new week, you have to go back to work, immerse yourself in the same arduous tasks from the previous week. The same goes for each new day. You have to shower, eat on time, do your chores, manage the home, keep family members happy, and follow so many other routine engagements just because another day has passed. The weekends and vacations are anticipated for the very reason that time loses its influence. The more the human mind can forget about the pressures that time brings, the more relaxed it will feel.

In bhakti, however, routine things that are monotonous can be turned around into pleasurable dependencies. The passage of time, the repetition of days, suddenly becomes a wonderful boon. The central component of the bhakti-yoga discipline is the chanting

 

 of the holy names, “Hare Krishna Hare Krishna, Krishna Krishna, Hare Hare, Hare Rama Hare Rama, Rama Rama, Hare Hare

 

”. The recommendation is that one chant this mantra for at least sixteen rounds a day on a set of japa beads. A japa mala consists of 108 beads, and chanting the mantra one time on each bead around the mala equates to one round. Therefore sixteen rounds means saying the mantra many, many times. Once finished with the daily routine, the same procedure must be repeated the next day, and the next, and so on.

But what if we didn’t have that routine, which is built on the passage of time as marked by the relative position of the sun? The opportunity for repeatedly reciting the holy name would go away. The chance to hear the sound of Krishna, which brings to mind the sweet vision of the Supreme Personality of Godhead smiling while holding His flute and giving that innocent glance that is both charming and inviting, would be missed. The most wonderful vision of the Supreme Lord in His form as Rama, holding a bow in His hands and waiting to defend and protect the innocent, receiving the service of Shri Hanuman

 

 and delighting in the company of Sita Devi

 

 and Lakshmana

 

, would remain far away from the mind.

Chanting is the foundation stone of bhakti-yoga, and it is meant to act as a springboard. From chanting comes hearing. From hearing comes the accumulation of thoughts, ideas for new ways to potentially connect with Krishna, the Supreme Absolute Truth. With other activities, such as visiting a temple, reading a book about Krishna, cooking nice food preparations and offering them to the Lord to become prasadam

 

, and singing along to kirtana songs, new aspects can be added to the routine.

From following a routine, habits develop. It is said that the habits one develops before they reach the age of thirty shape their behavior for the rest of their life. Therefore in the Vedic tradition, students are introduced to Krishna-bhakti as early as possible. There are other methods of self-realization, such as meditation, study of Vedanta, and fruitive work with the results renounced, but they each carry prerequisites. To meditate requires ideal conditions of peace and quiet, Vedanta study demands high intelligence, and fruitive work with detachment depends on knowledge of the impact of the work and the ability to carry out the functions properly.

All bhakti requires, however, is love. This love can be seen in even the child, so someone immature can take to chanting and dancing and be immersed in yoga. The aspect of spirituality that is applicable to the most number of people will be the best, and it will have God represented most fully. The holy name is non-different from Krishna, a truth proved by the fact that anyone can recite the holy name, even if they are unintelligent or unfamiliar with the principles of Vedic teachings.

From following bhakti, the dawn of each new day brings renewed hope, a chance to connect with Krishna again. The new day arrives without our desiring it, so this means that we will continue to get new opportunities in bhakti for as long as we shall so desire them. Just as the lotus flower opens at the sight of the splendorous sun, the sincere servant of the Supreme Lord wakes up every day with bright enthusiasm over their chance to tell their beloved just how much they love Him. At the end of life, that spiritual sunshine is met in His permanent home, with life’s mission fulfilled.

In Closing:

At rising of the bright sun we are glad,

But to repeat misery we are sad.

To man the sun is giver of light and heat,

But each day chores and tasks we must repeat.

Use the passing of days for your benefit,

So that best end you’ll meet after body to quit.

Daily chant maha-mantra rounds that are sixteen,

So that in your mind’s vision Krishna to be seen.

Take every day as giver of chance that is new,

To please the Lord of complexion dark blue.

Electricity Simplified via Simpa Networks

Paul Needham: Owning electricity

Paul Needham’s organization, Simpa Networks, makes solar energy available to the poor. By using a pay-as-you-go pricing structure modeled after mobile phone cards, Simpa gives its customers ownership of the electricity. Once the initial cost of the equipment is paid off, the device belongs to the customer and their electricity is free.

SUBMITTED BY PARVATHI MENON

This article was originally published on http://www.innovationalchemy.com/. Simpa Network has partned with SELCO, an India DM winner in 2011.

Simpa Networks has evolved a ‘Progressive Purchase‘ model for solar electricity, lighting up rural homes through a flexible payment option.  

The International Energy Agency estimates that about 1.5 billion people around the globe do NOT have access to electricity and 85% of these people live in rural areas.  In India,close to 40% of the country’s population still lives with limited access to grid electricity. This is not to say that rural India is in complete darkness. The up-front cost of procuring clean, affordable energy is high and so several parts of rural India rely on kerosene, charcoal and other forms of fuel that are easier to access and in local purchase terms, cheaper. The existence of these alternatives indicates that people have the ability to pay for energy, but it needs to be in a format and amount that they can access. Regular energy sources have not been able to find ways to fit this need yet. Simpa Networks leverages this insight into the rural market to find a way to fit within the ‘ability to pay’.

“Customers pay up to $1000 over 8-10 years for kerosene lanterns why not capture what the customer is willing to pay and give them a cleaner alternative?”says co-founder Michael Macharg.

Based in Bangalore, Simpa Networks aims to develop affordable energy solutions for the poor. Their product makes solar electricity accessible and affordable to the rural and under served consumer through their innovative pricing system called ‘Progressive Purchase’.

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Simpa Networks has developed a metered solar energy system that generates electricity and can be installed in any rural home. The system can be purchased for a small upfront cost and can be recharged (through local agents via SMS) according to usage. If the meter runs out, it switches off and comes back on once the user has paid for a recharge (recharge amounts vary from 50-500 rupees). But the real proposition is that as the user recharges the system, he or she is slowly paying to own the system. Once enough recharges have been made (across a period of approximately 3-5 years), the system unlocks and produces solar power for free.

What’s enabling adoption?

Flexibility. One of the reasons rural India relies on kerosene is that it appears cheaper since the user can pay only what he or she can afford, and buy based on money at hand. Simpa recognizes that rural incomes are irregular and applies this insight to their product pricing; the cost of electricity generated by the solar panels is the same as buying kerosene lamps. The ability to pay for clean electricity in small doses and the added bonus of eventually owning a solar panel that generates free power is what makes this product especially remarkable and innovative.

What are the challenges?

Need to create and build distribution and service channels into the targeted markets. Finding the right local partners to help distribute the solar panels into rural homes is a challenge. Right now, Simpa partners with SELCO India to take their product into villages and reach the rural population in Karnataka; and will need to find similar dedicated partners in other geographies to expand and scale up. The system also potentially requires routine maintenance. Servicing and employing an efficient workforce of partners to execute this maintenance is also a challenge that the Simpa team is looking to work through.

Impact – current and potential

Simpa Networks was incorporated in India in mid 2011 and have conducted their first round of pilot testing in Karnataka. They have a Sales agreement with SELCO India to sell 1000 solar home systems in 2012, growing to 5000 + systems through SELCO and other distributors in 2013. By 2014 they aim to have sold 25,000 solar home systems demonstrating a clear model and scale approach.

Paul Needham co-founder elaborates on the Simpa Business model. Watch him speak at Poptech 2011 (Video).

Products, services and business models that can help bring cheaper, more user friendly, clean energy into homes and work spaces across India is going to be a huge opportunity for innovators and entrepreneurs alike. Its early days yet, but enterprises such as Simpa Networks are doing some critical experiments in this direction and the insights from their learning and growth in this market will form the basis for a lot of development in this industry.

Follow Parvathi Menon on Twitter @parvathimenon and Innovation Alchemy @innovalchemy for regular updates.