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Tuesday, July 5, 2011

Biologists study how insect moms fight cannibalistic neighbors


Biologists study how insect moms fight cannibalistic neighbors

wood, there are mothers with problems. Assistant Professor of Biology Andy Zink and his students have published new research on the parenting dilemmas faced by maritime earwigs as they try to defend their nests against cannibalistic invaders.
A female maritime earwig with her eggs. Credit: Jonathan Wright
Maritime earwigs (Anisolabis maritima) live on beaches and are related to the European earwigs that are often seen in homes and yards. Along the high tide line, maritime earwigs make their nests in excavated chambers of sand or soil underneath rocks and logs, defending their eggs for three weeks until they hatch.
The problem for these dark, inch-long insects is that other female earwigs want to eat their un-hatched eggs, which are full of protein. This threat of egg cannibalism comes from their closest neighbors. Underneath a single log, there can be dozens of females, with nest chambers back to back like row houses.
“An invading female pokes her back end into the mother’s nest first. The backside is where their sharp forceps are,” said former graduate student Julie Miller. As part of her master’s thesis, Miller studied nest invasions in the lab, including video recordings of conflicts between females.
“The invading female earwig waves her forceps in an aggressive dance,” Miller said. “The two females then interlock forceps and grapple with one another. It’s usually the larger female that wins the contest, by chasing off or ripping a hole in the body of the smaller one.”
If the invading female wins the contest, her prize is being able to eat all of the nesting female’s eggs, usually about 40 eggs per nest. However, larger female nesters are able to repel invaders and keep their eggs alive. Zink and Miller’s findings suggest that the fighting required of mother earwigs could influence the evolution of larger body sizes among females. “Our analysis showed that larger females have an advantage over smaller females and are more likely to win the fight against an invader,” Miller said.
Mothers from all earwig species guard their eggs, but this is the first study to directly test if one of the benefits of maternal care is to prevent egg cannibalism. In lab experiments, Miller removed moms from nests and then introduced a female invader. She found that the mother’s presence was essential for preventing eggs from being eaten. Miller believes that maternal care may go hand in hand with maritime earwigs nesting so close to each other and that the risk of cannibalism may have influenced the evolution of parental care in this species and in other earwig species.
For Zink, the research raises interesting questions about the tradeoffs that female earwigs have to make. For example, a mother may flee the nest instead of fight, allowing her to reproduce again in a few days or weeks, or may eat her own eggs to bolster her strength for future reproduction.
“Studying these insects helps us understand the basic selective forces in nature that shape the evolution of parental care and social behavior in other animals, including humans,” Zink said. “The choices that earwigs have to make, to advocate for their offspring, mirror the tradeoffs that humans face around the costs and benefits of parenting.”
The findings were published online in May in the journal Behavioral Ecological Sociobiology. Zink and Miller co-authored the paper with undergraduate student Lena Rudolph.

DNA cages ‘can survive inside living cells’



Scientists at Oxford University have shown for the first time that molecular cages made from DNA can enter and survive inside living cells.
The work, a collaboration between physicists and molecular neuroscientists at Oxford, shows that artificial DNA cages that could be used to carry cargoes of drugs can enter living cells, potentially leading to new methods of drug delivery.
A report of the research is published online in the journal ACS Nano.
Human embryonic kidney cells were used to test the DNA cages
The cages developed by the researchers are made from four short strands of synthetic DNA. These strands are designed so that they naturally assemble themselves into a tetrahedron (a pyramid with four triangular faces) around 7 nanometres tall.
The Oxford researchers have previously shown that it is possible to assemble these cages around protein molecules, so that the protein is trapped inside, and that DNA cages can be programmed to open when they encounter specific ‘trigger’ molecules that are found inside cells.
In the new experiment they introduced fluorescently-labelled DNA tetrahedrons into human kidney cells grown in the laboratory. They then examined the cells under the microscope and found that the cages remained substantially intact, surviving attack by cellular enzymes, for at least 48 hours. This is a crucial advance: to be useful as a drug delivery vehicle, a DNA cage must enter cells efficiently and survive until it can release its cargo where and when it is needed.
‘At the moment we are only testing our ability to create and control cages made of DNA,’ said Professor Andrew Turberfield of Oxford University’s Department of Physics, who led the work. ‘However, these results are an important first step towards proving that DNA cages could be used to deliver cargoes, such as drugs, inside living cells.’
Professor Turberfield said: ‘Previous studies have shown that the size of particles is an important factor in whether or not they can easily enter cells, with particles with a radius less than 50 nanometres proving much more successful at gaining entry than larger particles. At 7 nanometres across our DNA tetrahedrons are compact enough to easily enter cells but still large enough to carry a useful cargo. More work is now needed to understand exactly how these DNA cages manage to find their way inside living cells.’

‘Cling-film’ solar cells could lead to advance in renewable energy



A scientific advance in renewable energy which promises a revolution in the ease and cost of using solar cells, has been announced today. A new study shows that even when using very simple and inexpensive manufacturing methods – where flexible layers of material are deposited over large areas like cling-film – efficient solar cell structures can be made.
A polymer solar cell ready for testing; the metal connections allow us to measure different areas of the film and measure the device efficiency amongst other parameters. Credit: Andrew Parnell
The study, published in the journal Advanced Energy Materials, paves the way for new solar cell manufacturing techniques and the promise of developments in renewable solar energy. Scientists from the Universities of Sheffield and Cambridge used the ISIS Neutron Source and Diamond Light Source at STFC Rutherford Appleton Laboratory in Oxfordshire to carry out the research.
Plastic (polymer) solar cells are much cheaper to produce than conventional silicon solar cells and have the potential to be produced in large quantities. The study showed that when complex mixtures of molecules in solution are spread onto a surface, like varnishing a table-top, the different molecules separate to the top and bottom of the layer in a way that maximises the efficiency of the resulting solar cell.
Dr Andrew Parnell of the University of Sheffield said, “Our results give important insights into how ultra-cheap solar energy panels for domestic and industrial use can be manufactured on a large scale. Rather than using complex and expensive fabrication methods to create a specific semiconductor nanostructure, high volume printing could be used to produce nanoscale (60 nanometers) films of solar cells that are over a thousand times thinner than the width of a human hair. These films could then be used to make cost-effective, light and easily transportable plastic solar cell devices such as solar panels.”
Dr Robert Dalgliesh, one of the ISIS scientists involved in the work, said, “This work clearly illustrates the importance of the combined use of neutron and X-ray scattering sources such as ISIS and Diamond in solving modern challenges for society. Using neutron beams at ISIS and Diamond’s bright X-rays, we were able to probe the internal structure and properties of the solar cell materials non-destructively. By studying the layers in the materials which convert sunlight into electricity, we are learning how different processing steps change the overall efficiency and affect the overall polymer solar cell performance.”
“Over the next fifty years society is going to need to supply the growing energy demands of the world’s population without using fossil fuels, and the only renewable energy source that can do this is the Sun”, said Professor Richard Jones of the University of Sheffield. “In a couple of hours enough energy from sunlight falls on the Earth to satisfy the energy needs of the Earth for a whole year, but we need to be able to harness this on a much bigger scale than we can do now. Cheap and efficient polymer solar cells that can cover huge areas could help move us into a new age of renewable energy.”
This image shows how neutrons are scattered from one of the solar cell layers. Modelling this information helps us understand the composition and structure within the layer. The intense horizontal line is the mirror-like reflection (specular reflectivity) from the solar cell. The data was taken on the instrument Offspec at ISIS' Target Station 2. Credit: STFC
Solar cells
Photovoltaics are semiconductor devices that are used to generate low-cost renewable energy – most commonly as solar panels. When sunlight hits a photovoltaic cell, it is absorbed and its energy is converted into an electrical current. Most photovoltaic devices are made with silicon; however, devices can also be made from plastic (organic photovoltaic devices).
Plastic films can be deposited from solution by low-cost, roll to roll printing techniques resulting in significant overall savings in energy and cost. This is where the film is put on a roll and goes through a series of processes similar to the way newspapers are printed and taken off a roll at the end. There are currently products using this type of technology. To increase usage further, however, the technology needs to be more efficient. Polymer solar cells are currently 7-8% efficient.The next step is to develop cells which are 10% efficient or more for commercial viability.
The materials used in the research carried out by the collaboration are called PCDTBT (poly [N-9′-heptadecanyl-2,7-carbazole-alt-5,5-(4′,7′-di- 2-thienyl- 2′,1′,3′-benzothiadiazole): PCBM ([6,6]- phenyl-C61-butyric acid methylester), a material based on Nobel-prize-winning (Chemistry 1996) work of Professor Richard Smalley and Professor Harry Kroto (amongst others) on the C60 Buckminsterfullerene or buckyball form of carbon.Bright X-rays using instruments at Diamond Light Source were used to study the crystallinity of the material; neutrons at ISIS were used to examine the material’s composition profile.

Surkra Gayatri Mantram

Surya Kavacha Stotram

Mahamritunjay Mantra

Chandra Graha Dhyana Slokam - Full

Guru Mantra

Sai Baba Mantra

Monday, July 4, 2011

A Supercomputer Waters the Lawn


Smart Irrigation: A Supercomputer Waters the Lawn

Satellite control of company and municipal irrigation systems, based on custom weather data, saves billions of gallons of water nationwide
Image: Flickr / Kumaravel

In Brief

Businesses and municipalities waste billions of gallons of water each year in poorly timed lawn irrigation.
Smart controllers on water lines, instructed by satellite, can deliver just the right amount of water at the most effective time.
HydroPoint's custom-programmed supercomputer models the daily water needs of every square kilometer across the U.S. and Canada, remotely driving clients' irrigation systems.
In Silicon Valley the Campbell Union School District's sprinklers used to dutifully water the soccer fields and gardens at 12 campuses even during spring showers. Temporarily shutting off each of the 45 irrigation control boxes, by hand, wasn't worth the custodians' time. But in 2009 the district installed new "smart" controllers that automatically adjust daily watering to the weather. Each box, fitted with a microprocessor and antenna, receives local real-time weather information by satellite from the WeatherTRAK climate center supercomputer run by Petaluma, Calif.–based HydroPoint Data Systems, Inc.

On one April morning after a three-day rainstorm, Campbell Union's facilities supervisor, David Radke, checked the status of the controllers by logging in toWeatherTRAK.net The service had put the district’s irrigation network on pause when the storm began, and according to WeatherTRAK's math, the network would not need to resume watering for eight to 11 days.

If needed, Radke can reprogram the controllers from his Web account, but because his crew has carefully customized each box's settings, he finds that "there's really not a whole lot to do. That's the beauty of it." The soccer fields look better than ever, yet in 2009 WeatherTRAK slashed Campbell Union's water use by 39 percent and cut its utility bills by $108,000, recouping the installation expenses nearly twice over.

With most sprinkler systems, property owners set the traditional controller—basically a timer—to irrigate at specific intervals. Often, too much water is lost to evaporation during hot weather or to runoff during cool weather, which can also carry chemicals into the local watershed or ocean. Because outdoor irrigation can suck up 50 percent or more of urban water consumption, smart irrigation services have caught on in drought-prone western states like California, where water prices are relentlessly rising. (Occasional big floods don't help the long-term problem.) HydroPoint now has more than 8,000 clients using 24,000 of its smart controllers, including Walmart, Coca-Cola, Hilton, Jack in the Box and the University of Arizona as well as the cities of Charleston, S.C., Houston and Santa Barbara. In 2011 customers are projected to save 64.4 billion liters of water and over $111 million in water expenses, as well as 68 million kilowatt-hours of water-pumping electricity.

The technology is still going through growing pains, but HydroPoint has solved several daunting weather-forecast and modeling challenges that help it deliver just the right amount of water at the most effective time.

Water by satellite
Founded in 2002 in Petaluma 65 kilometers north of San Francisco, HydroPoint has engineered what appears to be the most sophisticated weather-based irrigation system among a growing list of competitors. The company built its primary climate-modeling center outside of Salt Lake City, programming a supercomputer to simulate local weather for every square kilometer across North America—all just to water the grass. The center communicates via a two-way satellite link with control boxes that operate distinct zones of a client's irrigation system. Wires running underground from the outdoor boxes open and close valves in the water lines. Every night the climate center broadcasts local weather-related data to a microprocessor inside each controller, which runs software that uses the information to compute precisely how much and when to water its zones, customized to one of 18 plant types as well as other factors like soil type and ground slope.

The boxes can talk back, too. For example, Campbell Union installed additional wireless rain sensors (fixed to an exterior building wall) that, on detecting moisture, signals controllers, which put irrigation on hold and notify the climate center.

A WeatherTRAK prototype went live in 2003, starting with one-way satellite service in California. Two-way Internet-based communications became available in 2006, allowing a client to adjust thousands of controllers across multiple properties, all from one Web account. Monthly subscription fees today range from $4 to $18.75. The service is a prime example of the nascent field of water information systems that analyze real-time data to tackle water management issues—data that until recently has been sorely lacking in the industry. "You can't manage what you don't see," says HydroPoint co-founder Chris Spain.

Really local forecasts, in real time

Spain and his colleagues jumped into the business by buying a local irrigation-controller company and bringing on board its owner, Mike Marian, a self-taught engineer. Marian (who subsequently left HydroPoint in 2006) had patented the idea of using paging signals to transmit information to controllers about evapotranspiration(ET)—the amount of water soil loses to evaporation as well as transpiration by plantsgrowing there. Marian's system used free ET data from the nearest state weather stations, maintained for farmers by the California Irrigation Management Information System.

The entire state, however, had only around 120 stations. Simply averaging ET values from the two nearest stations (as farmers often did) could be misleading, because microclimates even a few kilometers apart can produce substantially different values. And although property managers could buy their own mini weather stations, those were less reliable.

The fledgling HydroPoint team instead boldly explored a cutting-edge alternative: using measurements from public weather stations across the U.S. to model an accurate ET for any location, down to a square kilometer—without having an actual station or sensors on-site. And doing it every night.

Dan Dansereau, a computer scientist who worked on high-resolution weather modeling at Utah State University in Logan thought it could be done. But he says he told Marian and Spain "You're going to need a big-ass computer," before he joined them as HydroPoint's chief scientist. Dansereau recalls one prominent colleague warning, "It's never going to work."

Indeed, modeling proved a complicated task. The team wanted to use the most accurate method for estimating ET, the Penman–Monteith equation, which factors in temperature, wind, solar radiation and relative humidity. But the trick to deriving real-time ET everywhere would be creating those four input parameters for each of the 19.2 million one-square-kilometer grids across the continental U.S. and Canada.

Dansereau's plan was to model the parameters using the same highly sophisticated (and public) forecasting software that the U.S. Air Force relied on, called theMesoscale model, or MM5, created by Pennsylvania State University and the National Center for Atmospheric Research (NCAR) in Boulder, Colo. Running MM5 at a daily one-square-kilometer resolution nationwide would take such tremendous computing power that no one had attempted it. Every day the climate center had to download millions of real-time data points via satellite from the National Oceanic and Atmospheric Administration (NOAA) and other public sources. That included surface observations collected hourly from ground weather stations, ocean buoys and weather balloons around the world, global satellite-imaging data every 15 minutes, and hourly Doppler radar data for the U.S. In addition, the MM5 program required 256 categories of local ground information such as topography, soil, vegetation and land use.

To manage the data avalanche, the HydroPoint engineers wrote proprietary code to organize and shovel it into databases. But they were still "really stymied" because MM5 required a lot of manual data entry, recalls co-founder and vice president Peter Carlson, who along with Dansereau literally typed in parameters every day. To streamline the process, they devised automated programs so the model could run itself from a single script.

Making forecasts accurate
A second major obstacle was verifying accuracy. How would the engineers know if WeatherTRAK's ET calculations were right? "This whole idea of 'ground truth' is a pretty complicated one," Spain says.

First, MM5 itself "was susceptible to garbage in–garbage out," Spain notes, because ground weather readings can themselves be wrong. The answer was writing a heuristic algorithm that crawls 24/7 through the incoming data and weeds out anomalous readings. For example, if the program detects an impossibly big swing in temperature or humidity readings from one hour to the next from the same weather station, that data is disqualified. Dansereau and Carlson later programmed similar verification steps into a more advanced successor to MM5, the Weather Research and Forecasting model (developed by NCAR, NOAA and partners), which the NationalWeather Service relies on. The result of HydroPoint's version of that model, which it runs today, is a "virtual weather station" for each square kilometer in North America. The outputs for temperature, wind, solar radiation and relative humidity are then funneled into the calculations of ET values, which are transmitted to customers' controllers by 8 P.M. Pacific time.

The second part of the accuracy problem was validating whether WeatherTRAK's numbers were on target. The engineers, focusing initially on California, realized they could check their daily results against actual ET measurements reported by the state's 120 weather-station array, which were published the following morning. "We process our data and publish the answers," Dansereau explains. "Then we get to check tomorrow to see how accurate our answers were." If WeatherTRAK finds an error or gets better information after the fact, it re-runs the model for past days, and can send retroactive corrections to controllers to continually ensure accuracy.

A glass half full—or more

A third key hurdle for HydroPoint was converting ET values into an irrigation schedule that would keep grass and plants in optimal health. The company developed scheduling software for the smart controllers that factors in ET plus 19 ground parameters entered by the client: from plant and soil types to sprinkler-head model. If a lawn's maximum water supply were the equivalent of a full glass of water, the goal is to let the level dwindle to half-full before topping the glass off again, Carlson says.

The WeatherTRAK system has continued to evolve. One ongoing challenge was rain. The company has always advised installing a rain switch that turns off the controllers when precipitation is detected. Since 2007 WeatherTRAK has also analyzed Doppler radar data to approximate the amount of rain that has accumulated on each square kilometer. The climate center roughly predicts the moisture depletion rate by subtracting the estimated daily ET loss for each grid, thus determining how long the irrigation pause should last—information that is sent to the controllers.

Supercomputing the savings
HydroPoint today runs WeatherTRAK on a blade  supercomputing cluster with two teraflops of number-crunching power. Jimy Dudhia, an atmospheric physicist at NCAR, who helped develop the Weather Research and Forecasting model, says it is surprising that HydroPoint can calculate real-time ET at such a fine-grain resolution across the U.S. The calculations require more computing power than the National Weather Service's 12-kilometer-resolution forecasts. "In terms of science, it's on the front end," Dudhia says.

Others also see ET modeling as the way to go. Managers of California Irrigation Management Information System, for example, have developed their own method for simulating ET values statewide. They post the free spatial data online, but it only covers California, and only at a two-square-kilometer resolution.

As with any weather modeling results, HydroPoint's won't be perfect. But does the computing footwork pay off? Michael Dukes, a University of Florida agricultural engineer who has studied various smart irrigation systems, says HydroPoint can be accurate under optimal conditions, but notes that other leading smart irrigation companies have also devised innovative solutions. For example, Rain Bird Corp.'s ET Manager controller—which, like HydroPoint, earned top scores on a standardized irrigation industry test—estimates ET from hourly data received wirelessly from private local weather stations. Dukes believes ET Manager might do a better job than WeatherTRAK of factoring in rainfall, using a tipping-bucket gauge on a client's site that quantifies precipitation more reliably than most rain sensors.

Still, HydroPoint can quote two dozen independent studies showing that WeatherTRAK curtailed water use by anywhere from 14 to 82 percent. But how much you save depends on how much you were wasting. A major study that tracked 2,294 California sites using 14 different brands of smart controllers found only a disappointing 6 percent average decline in water consumption in the first year. Nearly 57 percent of the sites saved water—but another 42 percent actually used more.

One explanation is that some landscapes were previously under-irrigated, so their usage rose, says civil engineer Peter Mayer of Aquacraft, a Boulder-based firm that conducted the California study. And if an irrigation system has leaky valves, high-end controllers may not help much. The study noted that HydroPoint's controllers, when averaged, reaped no savings the first year. Spain says that's because most of the devices were installed incorrectly. After Los Angeles's water agency fixed installation problems, the WeatherTRAK controllers reduced water use by roughly 16 percent.

The bottom line is that landscape contractors and property owners have to be educated about setting up and adjusting smart irrigation systems. Installation troubles are a real stumbling block, Mayer says. "We're kind of in the VCR-era of smart controllers, where nobody knows how to program it properly." Nevertheless, Mayer sees the evolving technology as a key conservation tool. The U.S. Environmental Protection Agency's Water Sense program plans to begin issuing labels for smart controller brands that pass efficiency certification by the end of this year.

From HydroPoint's perspective, the biggest barrier is the status quo of old habits that waste cheaply priced water. Now that rates are going up, attitudes are changing. The water crisis, Spain says, "is truly going to be the biggest issue of the century."

ABOUT THE AUTHOR(S)

Ingfei Chen is a freelance writer in the San Francisco Bay area. Her articles on neuroscience, medicine and ecology have appeared in The New York TimesSmithsonian and Science.